{"doi":"10.1101/2021.03.19.436090","title":"Limited effects of m <sup>6</sup> A modification on mRNA partitioning into stress granules","abstract":"ABSTRACT Recent studies have argued that the m 6 A modification of mRNAs promotes mRNA recruitment to stress granules through the interaction with YTHDF proteins (Anders et al., 2018; Ries et al., 2019). However, mRNAs that contain multiple m 6 A modified sites partition similarly into stress granules in both wild-type and m 6 A-deficient cells by single-molecule FISH suggesting m 6 A modifications play a minor role in mRNA partitioning into stress granules. Moreover, multiple linear regression analysis suggests m 6 A modification plays a minimal role in stress granule recruitment. Finally, the artificial tethering of 25 YTHDF proteins on reporter mRNAs leads to only a modest increase in mRNA partitioning to stress granules. These results indicate m 6 A modification makes a small, but measurable, contribution to recruiting specific mRNAs to stress granules.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":217293,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9481,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":287046,"name":"Tyler Matheny","orcid":null,"position":1,"is_corresponding":false},{"id":287047,"name":"Thao Ngoc Huynh","orcid":null,"position":2,"is_corresponding":false},{"id":814702,"name":"Vincent Babl","orcid":null,"position":3,"is_corresponding":false},{"id":234845,"name":"Roy Parker","orcid":"0000-0002-8412-4152","position":4,"is_corresponding":false},{"id":235311,"name":"Anthony Khong","orcid":"0000-0002-4293-8879","position":0,"is_corresponding":true}],"reference_count":27,"raw_metadata":null,"created_at":"2026-07-18T23:53:20.374789Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}