{"doi":"10.1101/2021.03.01.433480","title":"Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells","abstract":"ABSTRACT A successful HIV vaccine must overcome the hurdle of being able to activate naïve precursor B cells encoding features within their germline B cell receptors (BCR) that allow recognition of broadly neutralizing epitopes. Knowledge of whether broadly neutralizing antibody (bnAb) precursor B cells are circulating at sufficient frequencies within individuals in communities heavily impacted by HIV may be important. Using a germline-targeting eOD-GT8 immunogen and high-throughput droplet-based single cell BCR sequencing, we demonstrate that large numbers of paired BCR sequences from multiple donors can be efficiently screened to elucidate precursor frequencies of rare, naïve VRC01-class B cells. The results indicate that IGHV1-2 alleles incompatible with VRC01-class responses are relatively common in various human populations, and germline variation within IGHV1-2 associates with gene usage frequencies in the naïve BCR repertoire.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":213833,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":648397,"name":"Laura Toy","orcid":"0000-0002-4636-2731","position":1,"is_corresponding":false},{"id":490210,"name":"Justin T. Kos","orcid":"0000-0003-2645-3641","position":2,"is_corresponding":false},{"id":51320,"name":"Yana Safonova","orcid":"0000-0002-9634-4216","position":3,"is_corresponding":false},{"id":236279,"name":"William R. Schief","orcid":"0000-0002-1120-0150","position":4,"is_corresponding":false},{"id":51382,"name":"Corey T. Watson","orcid":"0000-0001-7248-8787","position":5,"is_corresponding":false},{"id":217933,"name":"Colin Havenar‐Daughton","orcid":"0000-0002-2880-3927","position":6,"is_corresponding":false},{"id":103780,"name":"Shane Crotty","orcid":"0000-0002-6484-6262","position":7,"is_corresponding":false},{"id":318058,"name":"Jeong Hyun Lee","orcid":"0000-0001-7371-845X","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-18T23:52:41.672172Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}