{"doi":"10.1101/2021.02.27.433115","title":"Single-cell analysis identifies a key role for\n                  <i>Hhip</i>\n                  in murine coronal suture development","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Craniofacial development depends on proper formation and maintenance of sutures between adjacent bones of the skull. In sutures, bone growth occurs at the edge of each bone, and suture mesenchyme maintains the separation between them. We performed single-cell RNA-seq analyses of the embryonic, murine coronal suture. Analyzing replicate libraries at E16.5 and E18.5, we identified 14 cell populations. Seven populations at E16.5 and nine at E18.5 comprised the suture mesenchyme, osteogenic cells, and associated populations. Through an integrated analysis with bulk RNA-seq data, we found a distinct coronal suture mesenchyme population compared to other neurocranial sutures, marked by expression of\n                  <jats:italic>Hhip</jats:italic>\n                  , an inhibitor of hedgehog signaling. We found that at E18.5,\n                  <jats:italic>\n                    Hhip\n                    <jats:sup>-/-</jats:sup>\n                  </jats:italic>\n                  coronal osteogenic fronts are closely apposed and suture mesenchyme is depleted, demonstrating that\n                  <jats:italic>Hhip</jats:italic>\n                  is required for coronal suture development. Our transcriptomic approach provides a rich resource for insight into normal and abnormal development.\n                </jats:p>","journal":null,"year":null,"id":647439,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":228082,"name":"Ana S. Gonzalez‐Reiche","orcid":"0000-0003-3583-4497","position":1,"is_corresponding":false},{"id":672673,"name":"Madrikha Saturne","orcid":"0000-0001-6677-5675","position":2,"is_corresponding":false},{"id":4390,"name":"Xianxiao Zhou","orcid":"0000-0001-9350-4467","position":3,"is_corresponding":false},{"id":672675,"name":"Ana Borges","orcid":"0000-0002-7787-6516","position":4,"is_corresponding":false},{"id":672676,"name":"Bhavana Shewale","orcid":"0000-0003-1243-7552","position":5,"is_corresponding":false},{"id":107090,"name":"Bin Zhang","orcid":"0000-0002-9549-5653","position":6,"is_corresponding":false},{"id":21492,"name":"Harm van Bakel","orcid":"0000-0002-1376-6916","position":7,"is_corresponding":false},{"id":308809,"name":"Ethylin Wang Jabs","orcid":"0000-0001-8983-5466","position":8,"is_corresponding":false},{"id":308802,"name":"Greg Holmes","orcid":"0000-0003-0717-6722","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Single-cell analysis identifies a key role for\n                  <i>Hhip</i>\n                  in murine coronal suture development","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Craniofacial development depends on proper formation and maintenance of sutures between adjacent bones of the skull. In sutures, bone growth occurs at the edge of each bone, and suture mesenchyme maintains the separation between them. We performed single-cell RNA-seq analyses of the embryonic, murine coronal suture. Analyzing replicate libraries at E16.5 and E18.5, we identified 14 cell populations. Seven populations at E16.5 and nine at E18.5 comprised the suture mesenchyme, osteogenic cells, and associated populations. Through an integrated analysis with bulk RNA-seq data, we found a distinct coronal suture mesenchyme population compared to other neurocranial sutures, marked by expression of\n                  <jats:italic>Hhip</jats:italic>\n                  , an inhibitor of hedgehog signaling. We found that at E18.5,\n                  <jats:italic>\n                    Hhip\n                    <jats:sup>-/-</jats:sup>\n                  </jats:italic>\n                  coronal osteogenic fronts are closely apposed and suture mesenchyme is depleted, demonstrating that\n                  <jats:italic>Hhip</jats:italic>\n                  is required for coronal suture development. Our transcriptomic approach provides a rich resource for insight into normal and abnormal development.\n                </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19910364","pmcid":null,"openalex_id":"https://openalex.org/W3134207939","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"5U01DE024448-05","title":"Transcriptome Atlases of the Craniofacial Sutures"},{"funder_name":"National Institutes of Health","grant_id":"1R03DE026814-01A1","title":"Multi-scale network biology approaches to analyze craniofacial expression data"},{"funder_name":"National Institutes of Health","grant_id":"1P01HD078233-01A1","title":"Project III: Systems Biology of Bone in Coronal Nonsyndromic Craniosynostosis"}],"total_grants":3,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"https://www.biorxiv.org/about/FAQ#license","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2021/02/27/2021.02.27.433115.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2021/02/27/2021.02.27.433115.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2021.02.27.433115","host_type":"publisher"},{"url":"https://doi.org/10.1101/2021.02.27.433115","host_type":"repository"},{"url":"https://dx.doi.org/10.1101/2021.02.27.433115","host_type":""}],"fields_of_study":["Craniofacial Disorders and Treatments","Congenital heart defects research","Developmental Biology and Gene Regulation","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Mesenchyme","Coronal suture","Calvaria","Fibrous joint","Biology","Cell biology","Coronal plane","Population","Anatomy","Mesenchymal stem cell","Medicine","Genetics"],"sdg_mappings":[{"sdg_number":10,"sdg_label":"10. 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