{"doi":"10.1101/2021.02.25.431980","title":"The plant immune receptors NRG1.1 and ADR1 are calcium influx channels","abstract":"Abstract Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. RPW8 domain-containing “helper” NLRs (RNLs) are required by many “sensor” NLRs. Our crystal structure of the RNL N REQUIREMENT GENE 1.1 (NRG1.1) N-terminal signaling domain resembled that of the resting state plant resistosome-forming HOPZ-ACTIVATED RESISTANCE 1 (ZAR1) and the animal MIXED-LINEAGE KINASE-LIKE (MLKL) cation channel. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta and conferred cytoplasmic Ca 2+ influx in plant and human HeLa cells. NRG1.1-dependent Ca 2+ influx and cell death were sensitive to Ca 2+ channel blockers. Ca 2+ influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another RNL, required conserved negatively charged N-terminal residues. Thus, RNLs apparently form influx channels to directly regulate cytoplasmic [Ca 2+ ] and consequent cell death. One Sentence Summary A specific class of plant immune receptors function as calcium-permeable channels upon activation to induce cell death.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":213949,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9598,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":617805,"name":"Nak Hyun Kim","orcid":"0000-0002-3722-1988","position":1,"is_corresponding":false},{"id":617806,"name":"Fei-Hua Wu","orcid":"0000-0002-5817-3831","position":2,"is_corresponding":false},{"id":30208,"name":"Farid El Kasmi","orcid":"0000-0002-4634-7689","position":3,"is_corresponding":false},{"id":245441,"name":"William G. Walton","orcid":"0000-0001-6745-534X","position":4,"is_corresponding":false},{"id":18790,"name":"Oliver J. Furzer","orcid":"0000-0002-3536-9970","position":5,"is_corresponding":false},{"id":617808,"name":"Adam D. Lietzan","orcid":"0000-0001-6388-2491","position":6,"is_corresponding":false},{"id":561952,"name":"Sruthi Sunil","orcid":"0000-0002-2142-5935","position":7,"is_corresponding":false},{"id":619435,"name":"Korina Kempthorn","orcid":null,"position":8,"is_corresponding":false},{"id":36347,"name":"Matthew R. Redinbo","orcid":"0000-0003-0814-5346","position":9,"is_corresponding":false},{"id":531861,"name":"Zhen‐Ming Pei","orcid":"0000-0003-1473-696X","position":10,"is_corresponding":false},{"id":74938,"name":"Li Wan","orcid":"0000-0002-0839-3748","position":11,"is_corresponding":false},{"id":18795,"name":"Jeffery L. Dangl","orcid":"0000-0003-3199-8654","position":12,"is_corresponding":false},{"id":617804,"name":"Pierre Jacob","orcid":"0000-0002-1253-3256","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-18T23:52:41.672172Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}