{"doi":"10.1101/2021.02.12.431035","title":"Computational anatomy and geometric shape analysis enables analysis of complex craniofacial phenotypes in zebrafish","abstract":"Abstract Due to the complexity of fish skulls, previous attempts to classify craniofacial phenotypes have relied on qualitative features or 2D landmarks. In this work we aim to identify and quantify differences in 3D craniofacial phenotypes in adult zebrafish mutants. We first estimate a synthetic ‘normative’ zebrafish template using microCT scans from a sample pool of wildtype animals using the Advanced Normalization Tools (ANTs). We apply a computational anatomy (CA) approach to quantify the phenotype of zebrafish with disruptions in bmp1a , a gene implicated in later skeletal development and whose human ortholog when disrupted is associated with Osteogenesis Imperfecta. Compared to controls, the bmp1a fish have larger otoliths and exhibit shape differences concentrated around the operculum, anterior frontal, and posterior parietal bones. Moreover, bmp1a fish differ in the degree of asymmetry. Our CA approach offers a potential pipeline for high throughput screening of complex fish craniofacial phenotypes, especially those of zebrafish which are an important model system for testing genome to phenome relationships in the study of development, evolution, and human diseases. Summary statement A computational anatomy approach offers a potential pipeline for high throughput screening of complex zebrafish craniofacial phenotypes, an important model system for the study of development, evolution, and human diseases.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":220241,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.6008,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":557935,"name":"Sara Rolfe","orcid":"0000-0001-9514-7774","position":1,"is_corresponding":false},{"id":313949,"name":"Ronald Y. Kwon","orcid":"0000-0001-9760-3761","position":2,"is_corresponding":false},{"id":557936,"name":"A. Murat Maga","orcid":"0000-0002-7921-9018","position":3,"is_corresponding":false},{"id":819774,"name":"Kelly Diamond","orcid":"0000-0001-8639-6795","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-18T23:53:46.965811Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}