{"doi":"10.1101/2021.02.03.429628","title":"Development of a highly sensitive bioanalytical assay for the quantification of favipiravir","abstract":"Favipiravir (FAV; T-705) has been approved for use as an anti-influenza therapeutic and has reports against a wide range of viruses (e.g., Ebola virus, rabies and norovirus). Most recently FAV has been reported to demonstrate activity against SARS-CoV-2. Repurposing opportunities have been intensively studied with only limited success to date. If successful, repurposing will allow interventions to become more rapidly available than development of new chemical entities. Pre-clinical and clinical investigations of FAV require robust, reproducible and sensitive bioanalytical assay. Here, a liquid chromatography tandem mass spectrometry assay is presented which was linear from 0.78-200 ng/mL Accuracy and precision ranged between 89% and 110%, 101% and 106%, respectively. The presented assay here has applications in both pre-clinical and clinical research and may be used to facilitate further investigations into the application of FAV against SARS-CoV-2.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":213943,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9504,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":254188,"name":"Megan Neary","orcid":"0000-0002-4960-2139","position":1,"is_corresponding":false},{"id":254184,"name":"Usman Arshad","orcid":"0000-0003-1586-1885","position":2,"is_corresponding":false},{"id":254185,"name":"Lee Tatham","orcid":"0000-0001-9448-8876","position":3,"is_corresponding":false},{"id":256856,"name":"Henry Pertinez","orcid":null,"position":4,"is_corresponding":false},{"id":256857,"name":"Helen Box","orcid":null,"position":5,"is_corresponding":false},{"id":254186,"name":"Rajith K. R. Rajoli","orcid":"0000-0002-6015-5712","position":6,"is_corresponding":false},{"id":256858,"name":"Anthony Valentijn","orcid":null,"position":7,"is_corresponding":false},{"id":254189,"name":"Joanne Sharp","orcid":"0000-0001-8482-5736","position":8,"is_corresponding":false},{"id":254192,"name":"Steve P. Rannard","orcid":"0000-0002-6946-1097","position":9,"is_corresponding":false},{"id":254201,"name":"Andrew Owen","orcid":"0000-0002-9819-7651","position":10,"is_corresponding":false},{"id":254187,"name":"Paul Curley","orcid":"0000-0003-4596-2708","position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":null,"created_at":"2026-07-18T23:52:41.672172Z","pmid":"33564761","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}