{"doi":"10.1101/2021.02.01.429250","title":"Molecular mechanism of N-terminal acetylation by the ternary NatC complex","abstract":"Abstract Protein N-terminal acetylation is predominantly a ribosome-associated modification, with NatA-E serving as the major enzymes. NatC is the most unusual of these enzymes, containing one Naa30 catalytic subunit and two auxiliary subunits, Naa35 and Naa38; and substrate specificity profile that overlaps with NatE. Here, we report the Cryo-EM structure of S. pombe NatC with a NatE/C-type bisubstrate analogue and inositol hexaphosphate (IP 6 ), and associated biochemistry studies. We find that the presence of three subunits is a prerequisite for normal NatC acetylation activity in yeast and that IP 6 binds tightly to NatC to stabilize the complex. We also describe the molecular basis for IP 6 -mediated NatC complex stabilization and the overlapping yet distinct substrate profiles of NatC and NatE.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":216394,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":468781,"name":"Leah Gottlieb","orcid":null,"position":1,"is_corresponding":false},{"id":379167,"name":"Buyan Pan","orcid":"0000-0003-1876-0326","position":2,"is_corresponding":false},{"id":699335,"name":"Julianna Supplee","orcid":"0000-0001-8234-7937","position":3,"is_corresponding":false},{"id":355182,"name":"Xuepeng Wei","orcid":"0000-0002-6514-7076","position":4,"is_corresponding":false},{"id":239973,"name":"E. James Petersson","orcid":"0000-0003-3854-9210","position":5,"is_corresponding":false},{"id":243334,"name":"Ronen Marmorstein","orcid":"0000-0003-4373-4752","position":6,"is_corresponding":false},{"id":277961,"name":"Sunbin Deng","orcid":"0000-0001-7798-4317","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:53:11.245932Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}