{"doi":"10.1101/2021.01.27.428417","title":"Microbiome-specific T follicular helper cells drive tertiary lymphoid structures and anti-tumor immunity against colorectal cancer","abstract":"Summary Colorectal cancer (CRC) is a common and deadly disease, and patients with metastatic tumors often fail to respond to therapy. While select members of the microbiome are associated with improved anti-tumor immunity, mechanistic understanding of how the microbiome provides a benefit is lacking. We show that modification of the CRC-associated microbiome with a single immunogenic commensal bacteria can alter T cell differentiation, inhibit tumor growth, and increase survival. Microbiome-driven control of CRC required the formation of colonic tertiary lymphoid structures (TLS) and increased infiltration of the tumor with cytotoxic immune cells. In the context of CRC, CD4 + T cells specific to the newly introduced commensals differentiated into T follicular helper cells and were necessary for the formation of TLS, immune infiltration of the tumor, and control over CRC. Thus, modification of the intestinal T cell response by the microbiome can be used to augment anti-tumor immunity in colorectal cancer.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":217141,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9516,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":109265,"name":"Anthony R. Cillo","orcid":"0000-0002-3213-3129","position":1,"is_corresponding":false},{"id":619896,"name":"Hannah J Bumgarner","orcid":null,"position":2,"is_corresponding":false},{"id":456902,"name":"Ansen B.P. Burr","orcid":"0000-0003-2524-897X","position":3,"is_corresponding":false},{"id":374383,"name":"Justin T. Tometich","orcid":"0000-0001-7402-4986","position":4,"is_corresponding":false},{"id":456903,"name":"Amrita Bhattacharjee","orcid":"0000-0001-5613-8963","position":5,"is_corresponding":false},{"id":109276,"name":"Tullia C. Bruno","orcid":"0000-0002-6433-0207","position":6,"is_corresponding":false},{"id":109278,"name":"Dario A.A. Vignali","orcid":"0000-0002-2771-5992","position":7,"is_corresponding":false},{"id":255655,"name":"Timothy W. Hand","orcid":"0000-0002-2684-9462","position":8,"is_corresponding":false},{"id":582282,"name":"Abigail E. Overacre-Delgoffe","orcid":"0000-0003-4027-3487","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-18T23:53:15.868165Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}