{"doi":"10.1101/2021.01.21.427626","title":"Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K <sup>+</sup> channels","abstract":"ABSTRACT Neuronal ankyrins cluster and link membrane proteins to the actin and spectrin-based cytoskeleton. Among the three vertebrate ankyrins, little is known about neuronal Ankyrin-R (AnkR). We report AnkR is highly enriched in Pv + fast-spiking interneurons in mouse and human. We identify AnkR-associated protein complexes including cytoskeletal proteins, cell adhesion molecules (CAMs), and perineuronal nets (PNNs). We show that loss of AnkR from forebrain interneurons reduces and disrupts PNNs, decreases anxiety-like behaviors, and changes the intrinsic excitability and firing properties of Pv + fast-spiking interneurons. These changes are accompanied by a dramatic reduction in Kv3.1b K + channels. We identify a novel AnkR-binding motif in Kv3.1b, and show that AnkR is both necessary and sufficient for Kv3.1b membrane localization in interneurons and at nodes of Ranvier. Thus, AnkR regulates Pv + fast-spiking interneuron function by organizing ion channels, CAMs, and PNNs, and linking these to the underlying β 1 spectrin-based cytoskeleton.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2021,"id":223051,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9606,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":813597,"name":"Colleen M Longley","orcid":"0000-0001-8326-6143","position":1,"is_corresponding":false},{"id":824209,"name":"Yuki Ogawa","orcid":"0000-0003-2126-9087","position":2,"is_corresponding":false},{"id":496048,"name":"Lindsay H. Teliska","orcid":"0000-0003-1733-6910","position":3,"is_corresponding":false},{"id":682891,"name":"Anithachristy S. Arumanayagam","orcid":null,"position":4,"is_corresponding":false},{"id":665008,"name":"Supna Nair","orcid":"0000-0001-6499-0099","position":5,"is_corresponding":false},{"id":275199,"name":"Juan A. Osés-Prieto","orcid":"0000-0003-4759-2341","position":6,"is_corresponding":false},{"id":256139,"name":"Alma L. Burlingame","orcid":"0000-0002-8403-7307","position":7,"is_corresponding":false},{"id":245007,"name":"Matthew D. Cykowski","orcid":"0000-0002-5973-803X","position":8,"is_corresponding":false},{"id":314833,"name":"Mingshan Xue","orcid":"0000-0003-1463-8884","position":9,"is_corresponding":false},{"id":275200,"name":"Matthew N. Rasband","orcid":"0000-0001-8184-2477","position":10,"is_corresponding":false},{"id":281243,"name":"Sharon R. Stevens","orcid":"0000-0003-2238-8029","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:54:07.287704Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}