{"doi":"10.1101/2020.12.24.424342","title":"Single cell multi-omic analysis identifies a <i>Tbx1</i> -dependent multilineage primed population in the murine cardiopharyngeal mesoderm","abstract":"Abstract The poles of the heart and branchiomeric muscles of the face and neck are formed from the cardiopharyngeal mesoderm (CPM) within the pharyngeal apparatus. The formation of the cardiac outflow tract and branchiomeric muscles are disrupted in patients with 22q11.2 deletion syndrome (22q11.2DS), due to haploinsufficiency of TBX1 , encoding a T-box transcription factor. Here, using single cell RNA-sequencing, we identified a multilineage primed population (MLP) within the CPM, marked by the Tbx1 lineage, which has bipotent properties to form cardiac and skeletal muscle cells. The MLPs are localized within the nascent mesoderm of the caudal lateral pharyngeal apparatus and provide a continuous source of progenitors that undergo TBX1-dependent progression towards maturation. Tbx1 also regulates the balance between MLP maintenance and maturation while restricting ectopic non-mesodermal gene expression. We further show that TBX1 confers this balance by direct regulation of MLP enriched genes and downstream pathways, partly through altering chromatin accessibility. Our study thus uncovers a new cell population and reveals novel mechanisms by which Tbx1 directs the development of the pharyngeal apparatus, which is profoundly altered in 22q11.2DS.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":131990,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9525,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":545496,"name":"Yang Liu","orcid":"0000-0002-5804-0043","position":1,"is_corresponding":false},{"id":586904,"name":"Christopher De Bono","orcid":"0000-0002-4330-7155","position":2,"is_corresponding":false},{"id":31857,"name":"Dario Righelli","orcid":"0000-0003-1504-3583","position":3,"is_corresponding":false},{"id":587445,"name":"Andrea Cirino","orcid":null,"position":4,"is_corresponding":false},{"id":497167,"name":"Wei Wang","orcid":"0009-0007-6722-2424","position":5,"is_corresponding":false},{"id":587446,"name":"Silvia E. Racedo","orcid":null,"position":6,"is_corresponding":false},{"id":586905,"name":"Anelisa Gollo Dantas","orcid":"0000-0003-0594-6762","position":7,"is_corresponding":false},{"id":586906,"name":"Chen‐Leng Cai","orcid":"0000-0001-6900-4737","position":8,"is_corresponding":false},{"id":586907,"name":"Claudia Angelini","orcid":"0000-0001-8350-8464","position":9,"is_corresponding":false},{"id":56523,"name":"Lionel Christiaen","orcid":"0000-0001-5930-5667","position":10,"is_corresponding":false},{"id":284565,"name":"Robert G. Kelly","orcid":"0000-0003-4357-0073","position":11,"is_corresponding":false},{"id":586908,"name":"Antonio Baldini","orcid":"0000-0002-5330-0256","position":12,"is_corresponding":false},{"id":284103,"name":"Deyou Zheng","orcid":"0000-0003-4354-5337","position":13,"is_corresponding":false},{"id":263880,"name":"Bernice E. Morrow","orcid":"0000-0002-8076-4726","position":14,"is_corresponding":false},{"id":587444,"name":"Hiroko Nomaru","orcid":null,"position":0,"is_corresponding":true}],"reference_count":86,"raw_metadata":null,"created_at":"2026-07-18T23:16:07.542484Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}