{"doi":"10.1101/2020.12.20.414748","title":"Cross-reactive coronavirus antibodies with diverse epitope specificities and extra-neutralization functions","abstract":"ABSTRACT The continual emergence of novel coronavirus (CoV) strains, like SARS-CoV-2, highlights the critical need for broadly reactive therapeutics and vaccines against this family of viruses. Coronavirus spike (S) proteins share common structural motifs that could be vulnerable to cross-reactive antibody responses. To study this phenomenon in human coronavirus infection, we applied a high-throughput sequencing method called LIBRA-seq (Linking B cell receptor to antigen specificity through sequencing) to a SARS-CoV-1 convalescent donor sample. We identified and characterized a panel of six monoclonal antibodies that cross-reacted with S proteins from the highly pathogenic SARS-CoV-1 and SARS-CoV-2 and demonstrated a spectrum of reactivity against other coronaviruses. Epitope mapping revealed that these antibodies recognized multiple epitopes on SARS-CoV-2 S, including the receptor binding domain (RBD), N-terminal domain (NTD), and S2 subunit. Functional characterization demonstrated that the antibodies mediated a variety of Fc effector functions in vitro and mitigated pathological burden in vivo . The identification of cross-reactive epitopes recognized by functional antibodies expands the repertoire of targets for pan-coronavirus vaccine design strategies that may be useful for preventing potential future coronavirus outbreaks.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":121057,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9582,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":559637,"name":"Kevin J. Kramer","orcid":"0000-0001-8451-9018","position":1,"is_corresponding":false},{"id":87305,"name":"Daniel Wrapp","orcid":"0000-0002-0538-9647","position":2,"is_corresponding":false},{"id":294732,"name":"Simone I. Richardson","orcid":"0000-0001-7678-2609","position":3,"is_corresponding":false},{"id":105112,"name":"Alexandra Schäfer","orcid":null,"position":4,"is_corresponding":false},{"id":559638,"name":"Steven C. Wall","orcid":"0000-0002-1220-7020","position":5,"is_corresponding":false},{"id":87306,"name":"Nianshuang Wang","orcid":"0000-0002-7569-011X","position":6,"is_corresponding":false},{"id":227360,"name":"Katarzyna Janowska","orcid":"0000-0002-7936-2244","position":7,"is_corresponding":false},{"id":559639,"name":"Kelsey A. Pilewski","orcid":"0000-0003-0916-4098","position":8,"is_corresponding":false},{"id":560086,"name":"Rohit Venkat","orcid":null,"position":9,"is_corresponding":false},{"id":229643,"name":"Rob Parks","orcid":null,"position":10,"is_corresponding":false},{"id":559640,"name":"Nelia P. Manamela","orcid":"0000-0002-2792-4815","position":11,"is_corresponding":false},{"id":560087,"name":"Nagarajan Raju","orcid":null,"position":12,"is_corresponding":false},{"id":559641,"name":"Emilee Friedman Fechter","orcid":"0000-0002-2250-3640","position":13,"is_corresponding":false},{"id":559642,"name":"Clinton Holt","orcid":"0000-0002-7635-0811","position":14,"is_corresponding":false},{"id":106506,"name":"Naveenchandra Suryadevara","orcid":"0000-0003-2571-1290","position":15,"is_corresponding":false},{"id":106500,"name":"Rita E. Chen","orcid":"0000-0002-5100-7750","position":16,"is_corresponding":false},{"id":105498,"name":"David R. Martinez","orcid":"0000-0002-2940-8961","position":17,"is_corresponding":false},{"id":106504,"name":"Rachel S. Nargi","orcid":null,"position":18,"is_corresponding":false},{"id":106505,"name":"Rachel E. Sutton","orcid":null,"position":19,"is_corresponding":false},{"id":218090,"name":"Julie E. Ledgerwood","orcid":"0000-0002-2047-6052","position":20,"is_corresponding":false},{"id":87311,"name":"Barney S. Graham","orcid":"0000-0001-8112-0853","position":21,"is_corresponding":false},{"id":249782,"name":"Michael S. Diamond","orcid":"0000-0002-6646-6781","position":22,"is_corresponding":false},{"id":96347,"name":"Barton F. Haynes","orcid":"0000-0002-7643-9023","position":23,"is_corresponding":false},{"id":227366,"name":"Priyamvada Acharya","orcid":"0000-0002-0089-277X","position":24,"is_corresponding":false},{"id":106530,"name":"Robert H. Carnahan","orcid":"0000-0001-5230-1532","position":25,"is_corresponding":false},{"id":106531,"name":"James E. Crowe","orcid":"0000-0002-0049-1079","position":26,"is_corresponding":false},{"id":5952,"name":"Ralph S. Baric","orcid":"0000-0001-6827-8701","position":27,"is_corresponding":false},{"id":36867,"name":"Lynn Morris","orcid":"0000-0003-3961-7828","position":28,"is_corresponding":false},{"id":87312,"name":"Jason S. McLellan","orcid":"0000-0003-3991-542X","position":29,"is_corresponding":false},{"id":290894,"name":"Ivelin S. Georgiev","orcid":"0000-0002-6312-7696","position":30,"is_corresponding":false},{"id":559636,"name":"Andrea R. Shiakolas","orcid":"0000-0001-9391-5434","position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:14:38.147936Z","pmid":"33398266","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}