{"doi":"10.1101/2020.12.18.423432","title":"A highly conserved binding pocket on PP2A-B56 is required for shugoshin binding and cohesion protection","abstract":"The shugoshin proteins are universal protectors of centromeric cohesin during mitosis and meiosis. The binding of human Sgo1 to the PP2A-B56 phosphatase through a coiled coil (CC) region is believed to mediate cohesion protection during mitosis. Here we undertook a structure function analysis of the PP2A-B56-Sgo1 complex, revealing unanticipated aspects of complex formation and function. We establish that a highly conserved pocket of the B56 regulatory subunit is required for Sgo1 binding and cohesion protection. Consistent with this, we show that Sgo1 blocks the binding of PP2A-B56 substrates containing a canonical B56 binding motif. Surprisingly, we identify B56 and Sgo1 mutants that prevent complex formation yet support cohesion protection and normal mitotic progression. This suggests that Sgo1 and PP2A-B56 have cohesion protection activity independently of complex formation. Collectively our work provides important insight into cohesion protection during mitosis.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":131915,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9513,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":586783,"name":"Michael A. Hadders","orcid":"0000-0002-2653-2625","position":1,"is_corresponding":false},{"id":586784,"name":"Melanie Weisser","orcid":"0000-0003-3946-3929","position":2,"is_corresponding":false},{"id":273954,"name":"Isha Nasa","orcid":"0000-0001-7699-795X","position":3,"is_corresponding":false},{"id":586785,"name":"Tanmay Gupta","orcid":"0000-0002-4882-0804","position":4,"is_corresponding":false},{"id":587385,"name":"Emil T Hertz","orcid":null,"position":5,"is_corresponding":false},{"id":274541,"name":"Thomas Kruse","orcid":"0000-0002-2619-7388","position":6,"is_corresponding":false},{"id":586786,"name":"Guillermo Montoya","orcid":"0000-0002-8269-3941","position":7,"is_corresponding":false},{"id":389689,"name":"A. Arockia Jeyaprakash","orcid":null,"position":8,"is_corresponding":false},{"id":273955,"name":"Arminja N. Kettenbach","orcid":"0000-0003-3979-4576","position":9,"is_corresponding":false},{"id":76202,"name":"Susanne M.A. Lens","orcid":"0000-0003-2199-7594","position":10,"is_corresponding":false},{"id":274550,"name":"Jakob Nilsson","orcid":"0000-0003-4100-1125","position":11,"is_corresponding":false},{"id":345393,"name":"Yumi Ueki","orcid":"0000-0003-2898-3267","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:16:07.542484Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}