{"doi":"10.1101/2020.12.16.422969","title":"SUMO-specific protease 2 (SENP2) suppresses browning of white adipose tissue through C/EBPβ modulation","abstract":"Abstract SUMO-specific protease 2 (SENP2) is highly expressed in white adipose tissue (WAT) and plays an important role in the early stages of adipogenesis. To investigate the function of SENP2 in adipocytes, we generated adipocyte-specific Senp2 knock-out ( Senp2 -aKO) mice. Compared to wild-type mice, Senp2 -aKO mice had reduced adipose tissue mass and smaller multi-locular adipocytes in inguinal WAT (iWAT). Body temperatures of Senp2 -aKO mice were effectively regulated during cold exposure. Additionally, Senp2 -aKO mice were resistant to high–fat–diet-induced obesity and insulin resistance and exhibited an increase in energy expenditure rates. Expression of thermogenic genes, including Ucp1 , was significantly increased in iWAT (and less efficiently in epidydimal WAT [eWAT]) of Senp2 -aKO mice, suggesting that SENP2 depletion accelerates browning of WAT. Further, suppression of HOXC10 was essential for beige adipocyte formation in SENP2-deficient cells of iWAT, and Hoxc10 transcriptional suppression was mediated by C/EBPβ, a direct target of SENP2. Sumoylated C/EBPβ efficiently inhibited Hoxc10 transcription through recruitment of the transcriptional co-repressor DAXX. Similarly, Senp2 knockdown using siRNAs during adipogenesis promoted thermogenic adipocyte differentiation of precursor cells in both iWAT and eWAT, and C/EBPβ was a common mediator. Together these results suggest that SENP2 plays critical role in white adipocyte differentiation by suppressing differentiation toward thermogenic adipocytes through modulation of C/EBPβ in both iWAT and eWAT.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":125678,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.964,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":572422,"name":"Sehyun Chae","orcid":"0000-0001-6904-0623","position":1,"is_corresponding":false},{"id":572423,"name":"Jinyan Nan","orcid":"0000-0001-9507-9943","position":2,"is_corresponding":false},{"id":572424,"name":"Young Do Koo","orcid":"0000-0002-9896-1023","position":3,"is_corresponding":false},{"id":485227,"name":"Seung‐Ah Lee","orcid":"0000-0002-9192-2849","position":4,"is_corresponding":false},{"id":283150,"name":"Young Joo Park","orcid":"0000-0002-3671-6364","position":5,"is_corresponding":false},{"id":32946,"name":"Daehee Hwang","orcid":"0000-0002-7553-0044","position":6,"is_corresponding":false},{"id":572425,"name":"Weiping Han","orcid":"0000-0002-5023-2104","position":7,"is_corresponding":false},{"id":298024,"name":"Young‐Bum Kim","orcid":"0000-0001-9471-6330","position":8,"is_corresponding":false},{"id":572426,"name":"Sung Soo Chung","orcid":"0000-0002-6017-0525","position":9,"is_corresponding":false},{"id":1103,"name":"Kyong Soo Park","orcid":"0000-0003-3597-342X","position":10,"is_corresponding":false},{"id":572421,"name":"Ji Seon Lee","orcid":"0000-0002-9769-7726","position":0,"is_corresponding":true}],"reference_count":63,"raw_metadata":null,"created_at":"2026-07-18T23:15:19.482428Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}