{"doi":"10.1101/2020.12.15.422962","title":"The six steps of the F <sub>1</sub> -ATPase rotary catalytic cycle","abstract":"Abstract F 1 F o ATP synthase interchanges phosphate transfer energy and proton motive force via a rotary catalysis mechanism. When isolated, its F 1 -ATPase catalytic core can hydrolyze ATP, rotating its γ rotor subunit. Although previous structural studies have contributed greatly to understanding rotary catalysis in F 1 , the structure of one major conformational state detected in single-molecule studies, termed the binding dwell state, has not yet been determined. Here, by exploiting a temperature-sensitive F 1 -ATPase mutant from Bacillus PS3, the structure of this binding dwell state was established together with that of the catalytic dwell state. Each state showed three catalytic β subunits in different conformations, providing the complete set of six β subunit conformational states taken up during catalysis cycle. These structures provide molecular details for the power-stroke conformational change that occurs upon ATP binding and induces a ~80° γ subunit rotation, as well as a second torque-generating conformational change, triggered by hydrolysis and product release, that produces a ~40° rotation. This study also identifies a putative phosphate-releasing tunnel that indicates how ADP and phosphate releasing steps are coordinated. Overall these findings provide a structural basis for the entire F 1 -ATPase rotary catalysis cycle.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":125684,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9579,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":572439,"name":"Hiroshi Ueno","orcid":"0000-0003-3991-0610","position":1,"is_corresponding":false},{"id":572440,"name":"Hiroyuki Noji","orcid":"0000-0002-8842-6836","position":2,"is_corresponding":false},{"id":267452,"name":"Alastair G. Stewart","orcid":"0000-0002-2070-6030","position":3,"is_corresponding":false},{"id":267448,"name":"Meghna Sobti","orcid":"0000-0001-7086-3467","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-18T23:15:19.482428Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}