{"doi":"10.1101/2020.12.11.420000","title":"A Synthetic Transcription Platform for Programmable Gene Expression in Mammalian Cells","abstract":"Abstract Precise, scalable, and sustainable control of genetic and cellular activities in mammalian cells is key to developing precision therapeutics and smart biomanufacturing. We created a highly tunable, modular, versatile CRISPR-based synthetic transcription system for the programmable control of gene expression and cellular phenotypes in mammalian cells. Genetic circuits consisting of well-characterized libraries of guide RNAs, binding motifs of synthetic operators, transcriptional activators, and additional genetic regulatory elements expressed mammalian genes in a highly predictable and tunable manner. We demonstrated the programmable control of reporter genes episomally and chromosomally, with up to 25-fold more activity than seen with the EF1α promoter, in multiple cell types. We used these circuits to program the secretion of human monoclonal antibodies and to control T-cell effector function marked by interferon-γ production. Antibody titers and interferon-γ concentrations significantly correlated with synthetic promoter strengths, providing a platform for programming gene expression and cellular function in diverse applications.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":124275,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.952,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":85526,"name":"Leonid Gaidukov","orcid":null,"position":1,"is_corresponding":false},{"id":85527,"name":"Yong Lai","orcid":"0000-0003-0208-4351","position":2,"is_corresponding":false},{"id":61644,"name":"Ming-Ru Wu","orcid":null,"position":3,"is_corresponding":false},{"id":75091,"name":"Jicong Cao","orcid":null,"position":4,"is_corresponding":false},{"id":85528,"name":"Michael J. Gutbrod","orcid":"0000-0003-0601-0077","position":5,"is_corresponding":false},{"id":75097,"name":"Gigi C. G. Choi","orcid":"0000-0003-2774-9188","position":6,"is_corresponding":false},{"id":85529,"name":"Rachel P. Utomo","orcid":null,"position":7,"is_corresponding":false},{"id":85532,"name":"Ying‐Chou Chen","orcid":"0000-0002-9404-2900","position":8,"is_corresponding":false},{"id":85533,"name":"Liliana Wróblewska","orcid":null,"position":9,"is_corresponding":false},{"id":103614,"name":"Manolis Kellis","orcid":"0000-0001-7113-9630","position":10,"is_corresponding":false},{"id":255357,"name":"Lin Zhang","orcid":"0000-0003-1998-0611","position":11,"is_corresponding":false},{"id":61653,"name":"Ron Weiss","orcid":"0000-0003-0396-2443","position":12,"is_corresponding":false},{"id":61655,"name":"Timothy K. Lu","orcid":"0000-0002-3918-8923","position":13,"is_corresponding":false},{"id":569422,"name":"William C.W. Chen","orcid":null,"position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-18T23:15:07.789881Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}