{"doi":"10.1101/2020.12.09.418384","title":"A plastid antiporter as a bioindicator of <i>Thalassiosira pseudonana</i> resilience","abstract":"ABSTRACT Acidification of the ocean due to high atmospheric CO 2 levels may increase the resilience of diatoms causing dramatic shifts in abiotic and biotic cycles with lasting implications on marine ecosystems. Here, we report a potential bioindicator of a shift in the resilience of a coastal and centric model diatom Thalassiosira pseudonana under elevated CO 2 . Specifically, we have discovered, through EGFP-tagging, a plastid membrane localized putative Na + (K + )/H + antiporter that is significantly upregulated at &gt; 800 ppm CO 2 , with a potentially important role in maintaining pH homeostasis. Notably, transcript abundance of this antiporter gene was relatively low and constant over the diel cycle under contemporary CO 2 conditions. In future acidified oceanic conditions, dramatic oscillations of &gt;10-fold change between nighttime (high) and daytime (low) in transcript abundances of the antiporter gene were associated with increased resilience of T. pseudonana . By analyzing metatranscriptomic data from the Tara Oceans project, we demonstrate that phylogenetically diverse diatoms express homologs of this antiporter across the globe. We propose that the differential between night- and daytime transcript levels of the antiporter could serve as a bioindicator of a shift in the resilience of diatoms in response to high CO 2 conditions in marine environments.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":127826,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9625,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":577378,"name":"J. R. Ashworth","orcid":"0000-0003-4835-6551","position":1,"is_corresponding":false},{"id":577379,"name":"Allison Cusick","orcid":"0000-0002-1372-2016","position":2,"is_corresponding":false},{"id":577380,"name":"Raffaela M. Abbriano","orcid":"0000-0002-5754-5461","position":3,"is_corresponding":false},{"id":577381,"name":"E. Virginia Armbrust","orcid":"0000-0001-7865-5101","position":4,"is_corresponding":false},{"id":256528,"name":"Mark Hildebrand","orcid":"0000-0003-0964-6628","position":5,"is_corresponding":false},{"id":577382,"name":"Mónica V. Orellana","orcid":"0000-0001-5990-9380","position":6,"is_corresponding":false},{"id":400246,"name":"Nitin S. Baliga","orcid":"0000-0001-9157-5974","position":7,"is_corresponding":false},{"id":577377,"name":"Jacob J. Valenzuela","orcid":"0000-0002-2320-241X","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-18T23:15:34.966380Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}