{"doi":"10.1101/2020.12.01.406140","title":"GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate","abstract":"Abstract GATA4/5/6 transcription factors play essential, conserved roles in heart development. How these factors mediate the transition from multipotent mesoderm progenitors to a committed cardiac fate is unclear. To understand how GATA4/5/6 modulate cell fate decisions we labelled, isolated, and performed single-cell gene expression analysis on cells that express gata5 at pre-cardiac time points spanning gastrulation to somitogenesis. We found that most mesendoderm-derived lineages had dynamic gata5/6 expression. In the absence of Gata5/6, the population structure of mesendoderm-derived cells was dramatically altered. In addition to the expected absence of cardiac mesoderm, we observed a concomitant expansion of cranial-pharyngeal mesoderm. Functional genetic analyses in zebrafish and the invertebrate chordate Ciona , which possess a single GATA4/5/6 homolog, revealed an essential and cell-autonomous role for GATA4/5/6 in promoting cardiac and inhibiting pharyngeal mesoderm identity. Overall, the maintenance and repression of GATA4/5/6 activity plays a critical, evolutionarily conserved role in early development.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":122404,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.955,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":334979,"name":"Xuefei Yuan","orcid":"0000-0001-5193-9964","position":1,"is_corresponding":false},{"id":563523,"name":"Claudia Racioppi","orcid":"0000-0001-8117-1124","position":2,"is_corresponding":false},{"id":563524,"name":"Meaghan Leslie","orcid":"0000-0003-0977-8268","position":3,"is_corresponding":false},{"id":564107,"name":"Anastasiia Aleksandrova","orcid":null,"position":4,"is_corresponding":false},{"id":56523,"name":"Lionel Christiaen","orcid":"0000-0001-5930-5667","position":5,"is_corresponding":false},{"id":79291,"name":"Michael D. Wilson","orcid":"0000-0002-4015-3066","position":6,"is_corresponding":false},{"id":334989,"name":"Ian C. Scott","orcid":"0000-0001-6665-1410","position":7,"is_corresponding":false},{"id":563522,"name":"Mengyi Song","orcid":"0000-0002-6112-8521","position":0,"is_corresponding":true}],"reference_count":106,"raw_metadata":null,"created_at":"2026-07-18T23:14:51.076430Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}