{"doi":"10.1101/2020.11.29.402735","title":"Microfluidic Device Facilitates Novel <i>In Vitro</i> Modeling of Human Neonatal Necrotizing Enterocolitis-on-a-Chip","abstract":"Abstract Necrotizing enterocolitis (NEC) is a deadly gastrointestinal disease of premature infants characterized by an exaggerated inflammatory response, dysbiosis of the gut microbiome, decreased epithelial cell proliferation, and gut barrier disruption. Here, we describe a novel in vitro model of human neonatal small intestinal epithelium (Neonatal-Intestine-on-a-Chip) that mimics key features of intestinal physiology by utilizing a combination of premature infant intestinal enteroids co-cultured with human intestinal microvascular endothelial cells within a microfluidic device. We used our Neonatal-Intestine-on-a-Chip to recapitulate NEC pathophysiology in an in vitro model system of the premature gut inoculated with infant-derived microbiota. This model, also known as NEC-on-a-Chip, emulates the prominent features of NEC, demonstrating significant upregulation of pro-inflammatory cytokines, decreased intestinal epithelial cell markers, reduced epithelial proliferation and disrupted epithelial barrier integrity. NEC-on-a-Chip provides a novel preclinical model of NEC, which may be used as a personalized medicine approach to test new therapeutics for this devastating disease.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123154,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9444,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":368998,"name":"Cliff J. Luke","orcid":"0000-0002-7408-2374","position":1,"is_corresponding":false},{"id":273934,"name":"Lila S. Nolan","orcid":"0000-0002-4185-2686","position":2,"is_corresponding":false},{"id":504597,"name":"Qingqing Gong","orcid":"0009-0003-2425-1701","position":3,"is_corresponding":false},{"id":276226,"name":"Jamie M. Rimer","orcid":null,"position":4,"is_corresponding":false},{"id":469142,"name":"Sarah E. Gale","orcid":"0000-0001-8762-4497","position":5,"is_corresponding":false},{"id":566531,"name":"Raymond Luc","orcid":null,"position":6,"is_corresponding":false},{"id":566532,"name":"Shay S. Bidani","orcid":null,"position":7,"is_corresponding":false},{"id":566533,"name":"Carrie A. Sibbald","orcid":null,"position":8,"is_corresponding":false},{"id":566534,"name":"Angela N. Lewis","orcid":null,"position":9,"is_corresponding":false},{"id":505567,"name":"Belgacem Mihi","orcid":null,"position":10,"is_corresponding":false},{"id":504599,"name":"Pranjal Agrawal","orcid":"0000-0002-9952-4888","position":11,"is_corresponding":false},{"id":565803,"name":"Martin Goree","orcid":"0000-0002-9871-1737","position":12,"is_corresponding":false},{"id":565804,"name":"Marlie M. Maestas","orcid":"0000-0003-4396-9770","position":13,"is_corresponding":false},{"id":565805,"name":"Elise Hu","orcid":"0000-0002-2497-9458","position":14,"is_corresponding":false},{"id":504601,"name":"David G. Peters","orcid":"0000-0003-4354-1125","position":15,"is_corresponding":false},{"id":273935,"name":"Misty Good","orcid":"0000-0002-0264-5721","position":16,"is_corresponding":false},{"id":463735,"name":"Wyatt E. Lanik","orcid":"0000-0002-8682-2931","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-18T23:14:59.547352Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}