{"doi":"10.1101/2020.11.24.396325","title":"Spatial control of gene expression in flies using bacterially derived binary transactivation systems","abstract":"Abstract Controlling gene expression is an instrumental tool for biotechnology, as it enables the dissection of gene function, affording precise spatial-temporal resolution. To generate this control, binary transactivational systems have been used employing a modular activator consisting of a DNA binding domain(s) fused to activation domain(s). For fly genetics, many binary transactivational systems have been exploited in vivo ; however as the study of complex problems often requires multiple systems that can be used in parallel, there is a need to identify additional bipartite genetic systems. To expand this molecular genetic toolbox, we tested multiple bacterially-derived binary transactivational systems in Drosophila melanogaster including the p -CymR operon from Pseudomonas putida , PipR operon from Streptomyces coelicolor , TtgR operon from Pseudomonas putida , and the VanR operon from Caulobacter crescentus . Our work provides the first characterization of these systems in an animal model in vivo . For each system we demonstrate robust tissue-specific spatial transactivation of reporter gene expression, enabling future studies to exploit these transactivational systems for molecular genetic studies.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":127721,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9477,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":577171,"name":"Luis C. Vesga","orcid":"0000-0002-0882-0420","position":1,"is_corresponding":false},{"id":577172,"name":"Stelia C. Méndez‐Sánchez","orcid":"0000-0002-2402-3632","position":2,"is_corresponding":false},{"id":262214,"name":"Omar S. Akbari","orcid":"0000-0002-6853-9884","position":3,"is_corresponding":false},{"id":262207,"name":"Stephanie Gamez","orcid":"0000-0003-0665-0066","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-18T23:15:34.966380Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}