{"doi":"10.1101/2020.11.17.387829","title":"Design to Data for mutants of β-glucosidase B from <i>Paenibacillus polymyxa:</i> L171M, H178M, M221L, E406W, N160E, F415M","abstract":"ABSTRACT Computational protein design is growing in popularity as a means to engineer enzymes. Currently, protein design algorithms can predict the stability and function of the enzymes to only a limited degree. Thus, further experimental data is required for training software to more accurately characterize the structure-function relationship of enzymes. To date, the Design2Data (D2D) database holds 129 single point mutations of β-glucosidase B (BglB) characterized by kinetic and thermal stability biophysical parameters. In this study, we introduced six mutants into the BglB database and examined their catalytic activity and thermal stability: L171M, H178M, M221L, E406W, N160E, and F415M.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":127643,"datarank":0.12439031925666323,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.02041824217267142,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.02041824217267142,"corpus_percentile":28.846600139243446,"corpus_rank":9199,"citation_count":1,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.9107,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":356613,"name":"Daniel Seung Kim","orcid":"0000-0003-2971-1909","position":1,"is_corresponding":false},{"id":577027,"name":"Peishan Huang","orcid":"0000-0002-8417-5793","position":2,"is_corresponding":false},{"id":577028,"name":"Ashley Vater","orcid":"0000-0001-6003-5086","position":3,"is_corresponding":false},{"id":298489,"name":"Justin B. Siegel","orcid":"0000-0003-1782-4076","position":4,"is_corresponding":false},{"id":577026,"name":"Xiaoqing Huang","orcid":"0009-0003-2183-8398","position":0,"is_corresponding":true}],"reference_count":16,"raw_metadata":null,"created_at":"2026-07-18T23:15:34.966380Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}