{"doi":"10.1101/2020.11.17.386532","title":"The Development of a Novel Nanobody Therapeutic for SARS-CoV-2","abstract":"Abstract Combating the COVID-19 pandemic requires potent and low-cost therapeutics. We identified a novel series of single-domain antibodies (i.e., nanobody), Nanosota-1, from a camelid nanobody phage display library. Structural data showed that Nanosota-1 bound to the oft-hidden receptor-binding domain (RBD) of SARS-CoV-2 spike protein, blocking out viral receptor ACE2. The lead drug possessing an Fc tag ( Nanosota-1C-Fc ) bound to SARS-CoV-2 RBD with a K d of 15.7picomolar (∼3000 times more tightly than ACE2 did) and inhibited SARS-CoV-2 infection with an ND 50 of 0.16microgram/milliliter (∼6000 times more potently than ACE2 did). Administered at a single dose, Nanosota-1C-Fc demonstrated preventive and therapeutic efficacy in hamsters subjected to SARS-CoV-2 infection. Unlike conventional antibody drugs, Nanosota-1C-Fc was produced at high yields in bacteria and had exceptional thermostability. Pharmacokinetic analysis of Nanosota-1C-F c documented a greater than 10-day in vivo half-life efficacy and high tissue bioavailability. Nanosota-1C-Fc is a potentially effective and realistic solution to the COVID-19 pandemic. Impact statement Potent and low-cost Nanosota-1 drugs block SARS-CoV-2 infections both in vitro and in vivo and act both preventively and therapeutically.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":119213,"datarank":0.4887144807032224,"base_score":3.258096538021482,"endowment":3.258096538021482,"self_citation_contribution":0.4887144807032224,"citation_network_contribution":0.0,"self_endowment_contribution":0.4887144807032224,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9504,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":310679,"name":"Joseph P. Gallant","orcid":"0000-0003-4943-1744","position":1,"is_corresponding":false},{"id":428539,"name":"Christopher Massey","orcid":"0000-0002-2396-8909","position":2,"is_corresponding":false},{"id":103757,"name":"Ke Shi","orcid":"0000-0003-4175-3714","position":3,"is_corresponding":false},{"id":255554,"name":"Wanbo Tai","orcid":"0000-0002-9864-8993","position":4,"is_corresponding":false},{"id":227005,"name":"Jian Zheng","orcid":"0000-0003-0909-128X","position":5,"is_corresponding":false},{"id":553733,"name":"Abby Odle","orcid":"0000-0003-0526-9503","position":6,"is_corresponding":false},{"id":553734,"name":"Molly A. Vickers","orcid":"0000-0003-3796-5730","position":7,"is_corresponding":false},{"id":103704,"name":"Jian Shang","orcid":"0000-0003-2600-6059","position":8,"is_corresponding":false},{"id":103703,"name":"Yushun Wan","orcid":"0000-0003-0496-6315","position":9,"is_corresponding":false},{"id":255555,"name":"Aleksandra Drelich","orcid":"0000-0002-0201-5017","position":10,"is_corresponding":false},{"id":554907,"name":"Kempaiah R. Kempaiah","orcid":null,"position":11,"is_corresponding":false},{"id":553735,"name":"Vivian Tat","orcid":"0009-0002-0274-3900","position":12,"is_corresponding":false},{"id":77538,"name":"Stanley Perlman","orcid":"0000-0003-4213-2354","position":13,"is_corresponding":false},{"id":105109,"name":"Lanying Du","orcid":"0000-0001-5955-1294","position":14,"is_corresponding":false},{"id":218437,"name":"Chien‐Te K. Tseng","orcid":"0000-0001-5726-9834","position":15,"is_corresponding":false},{"id":103759,"name":"Hideki Aihara","orcid":"0000-0001-7508-6230","position":16,"is_corresponding":false},{"id":310681,"name":"Aaron M. LeBeau","orcid":"0000-0003-3802-7789","position":17,"is_corresponding":false},{"id":103706,"name":"Fang Li","orcid":"0000-0002-1958-366X","position":18,"is_corresponding":false},{"id":103756,"name":"Gang Ye","orcid":"0000-0001-6034-2174","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:14:08.313144Z","pmid":"33236012","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}