{"doi":"10.1101/2020.11.10.377382","title":"Distributed Representation of Taste Quality by Second-Order Gustatory Neurons in <i>Drosophila</i>","abstract":"SUMMARY Sweet and bitter compounds excite different sensory cells and drive opposing behaviors. It is commonly thought that the neural circuits linking taste sensation to behavior conform to a labeled-line architecture, but in Drosophila , evidence for labeled lines beyond first-order neurons is lacking. To address this, we devised trans- Tango(activity), a strategy for calcium imaging of second-order gustatory projection neurons based on trans -Tango, a genetic transsynaptic tracing technique. We found distinct projection neuron populations that respond to sweet and bitter tastants. However, the bitter-responsive population was also activated by water alone. We further discovered that bitter tastants evoke activity upon both stimulus onset and offset. Bitter offset responses are exhibited by both first- and second-order gustatory neurons, but these responses are distributed among multiple types of projection neurons in the second order. These findings suggest a more complex coding scheme for gustatory information than can be explained by a labeled line model.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":120569,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9491,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":558507,"name":"John D. Fisher","orcid":"0000-0003-2748-8715","position":1,"is_corresponding":false},{"id":65353,"name":"Griffin G. Hartmann","orcid":"0000-0001-9213-8995","position":2,"is_corresponding":false},{"id":334732,"name":"Mustafa Talay","orcid":"0000-0002-9046-1366","position":3,"is_corresponding":false},{"id":15471,"name":"Gilad Barnea","orcid":"0000-0001-6842-3454","position":4,"is_corresponding":false},{"id":558506,"name":"Nathaniel J. Snell","orcid":"0000-0002-8033-366X","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-18T23:14:38.147936Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}