{"doi":"10.1101/2020.10.27.357533","title":"M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity","abstract":"ABSTRACT Genome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization. Genome-wide, M1BP specifically colocalizes with CP190 at Motif 1-containing promoters, which are enriched at topologically associating domain (TAD) borders. M1BP facilitates CP190 chromatin binding at many shared sites and vice versa. Both factors promote Motif 1-dependent gene expression and transcription near TAD borders genome-wide. Finally, loss of M1BP reduces chromatin accessibility and increases both inter- and intra-TAD local genome compaction. Our results reveal physical and functional interaction between CP190 and M1BP to activate transcription at TAD borders and mediate chromatin insulator-dependent genome organization.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":122350,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9616,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":563358,"name":"Shue Chen","orcid":"0000-0002-0061-8041","position":1,"is_corresponding":false},{"id":563359,"name":"Leah F. Rosin","orcid":"0000-0002-2489-4016","position":2,"is_corresponding":false},{"id":504216,"name":"Yang Chen","orcid":"0000-0003-0147-3443","position":3,"is_corresponding":false},{"id":265463,"name":"Chen-Yu Liu","orcid":null,"position":4,"is_corresponding":false},{"id":564064,"name":"Guo-Yun Yu","orcid":null,"position":5,"is_corresponding":false},{"id":563360,"name":"Elissa P. Lei","orcid":"0000-0003-4894-0037","position":6,"is_corresponding":false},{"id":563357,"name":"Indira Bag","orcid":"0000-0002-5491-5869","position":0,"is_corresponding":true}],"reference_count":74,"raw_metadata":null,"created_at":"2026-07-18T23:14:51.076430Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}