{"doi":"10.1101/2020.10.22.350447","title":"The Histone Chaperone CAF-1 Sustains Myeloid Lineage Identity","abstract":"Abstract During hematopoiesis, stem and progenitor cells become progressively restricted in their differentiation potential. This process is driven by lineage-specific transcription factors and is accompanied by dynamic changes in chromatin structure. The chromatin assembly factor complex CAF-1 is a key regulator of cellular plasticity in various cell lineages in different organisms. However, whether CAF-1 sustains lineage identity during normal homeostasis is unclear. To address this question, we investigated the role of CAF-1 in myeloid progenitor cells. CAF-1 suppression in myeloid progenitors triggered their rapid commitment but incomplete differentiation toward granulocyte, megakaryocyte, and erythrocyte lineages, resulting in a mixed cellular state. Through comparison with a canonical paradigm of directed terminal myeloid differentiation, we define changes in chromatin accessibility that underlie a unique transcriptome of the aberrantly matured CAF-1 deficient cells. We further identify C/EBPα and ELF1 as key transcription factors whose control of myeloid lineage commitment is kept in check by CAF-1. These findings shed new light on molecular underpinnings of hematopoiesis and suggest that manipulation of chromatin accessibility through modulating CAF-1 levels may provide a powerful strategy for controlled differentiation of blood cells.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":125502,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9407,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":339728,"name":"Fei Ji","orcid":"0000-0002-7120-3812","position":1,"is_corresponding":false},{"id":571614,"name":"Jernej Murn","orcid":"0000-0002-1729-5859","position":2,"is_corresponding":false},{"id":274018,"name":"David Frankhouser","orcid":"0000-0002-8233-5853","position":3,"is_corresponding":false},{"id":327064,"name":"M. Andrés Blanco","orcid":"0000-0001-5057-4334","position":4,"is_corresponding":false},{"id":572101,"name":"Carmen Chiem","orcid":null,"position":5,"is_corresponding":false},{"id":571615,"name":"MiHyun Jang","orcid":"0000-0001-5638-2237","position":6,"is_corresponding":false},{"id":260528,"name":"Ruslan I. Sadreyev","orcid":"0000-0002-4240-5466","position":7,"is_corresponding":false},{"id":274023,"name":"Russell C. Rockne","orcid":"0000-0002-1557-159X","position":8,"is_corresponding":false},{"id":91625,"name":"David B. Sykes","orcid":"0000-0002-9788-0221","position":9,"is_corresponding":false},{"id":56485,"name":"Konrad Hochedlinger","orcid":"0000-0001-5811-5386","position":10,"is_corresponding":false},{"id":571616,"name":"Sihem Cheloufi","orcid":"0000-0002-1726-4796","position":11,"is_corresponding":false},{"id":454972,"name":"Yiming Guo","orcid":"0000-0002-4212-3784","position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":null,"created_at":"2026-07-18T23:15:15.482227Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}