{"doi":"10.1101/2020.10.14.338699","title":"Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels","abstract":"Abstract Ultrasound has been used to non-invasively manipulate neuronal functions in humans and other animals 1–4 . However, this approach is limited as it has been challenging to target specific cells within the brain or body 5–8 . Here, we identify human Transient Receptor Potential A1 ( hs TRPA1) as a candidate that confers ultrasound sensitivity to mammalian cells. Ultrasound-evoked gating of hs TRPA1 specifically requires its N-terminal tip region and cholesterol interactions; and target cells with an intact actin cytoskeleton, revealing elements of the sonogenetic mechanism. Next, we use calcium imaging and electrophysiology to show that hs TRPA1 potentiates ultrasound-evoked responses in primary neurons. Furthermore, unilateral expression of hs TRPA1 in mouse layer V motor cortical neurons leads to c-fos expression and contralateral limb responses in response to ultrasound delivered through an intact skull. Collectively, we demonstrate that hs TRPA1-based sonogenetics can effectively manipulate neurons within the intact mammalian brain, a method that could be used across species.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":120811,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9493,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":559086,"name":"Corinne A. Lee-Kubli","orcid":"0000-0001-5877-7312","position":1,"is_corresponding":false},{"id":559876,"name":"Yusuf Tufail","orcid":null,"position":2,"is_corresponding":false},{"id":559087,"name":"Uri Magaram","orcid":"0000-0001-8324-0437","position":3,"is_corresponding":false},{"id":559088,"name":"Janki Patel","orcid":"0000-0002-8140-9347","position":4,"is_corresponding":false},{"id":559089,"name":"Ahana Chakraborty","orcid":"0000-0001-8667-4062","position":5,"is_corresponding":false},{"id":559090,"name":"Jose Mendoza Lopez","orcid":"0000-0002-0711-5412","position":6,"is_corresponding":false},{"id":559091,"name":"Eric Edsinger","orcid":"0000-0002-1012-1506","position":7,"is_corresponding":false},{"id":559092,"name":"Aditya Vasan","orcid":"0000-0002-6329-0397","position":8,"is_corresponding":false},{"id":559877,"name":"Rani Shiao","orcid":null,"position":9,"is_corresponding":false},{"id":559093,"name":"Connor Weiss","orcid":"0000-0002-2597-9636","position":10,"is_corresponding":false},{"id":415577,"name":"James Friend","orcid":"0000-0003-0416-2165","position":11,"is_corresponding":false},{"id":559094,"name":"Sreekanth H. Chalasani","orcid":"0000-0003-2522-8338","position":12,"is_corresponding":false},{"id":559085,"name":"Marc Duque","orcid":"0000-0003-2999-4283","position":0,"is_corresponding":true}],"reference_count":83,"raw_metadata":null,"created_at":"2026-07-18T23:14:38.147936Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}