{"doi":"10.1101/2020.10.13.336008","title":"<i>In Situ</i> Optical Quantification of Extracellular Electron Transfer using Plasmonic Metal Oxide Nanocrystals","abstract":"Abstract Extracellular electron transfer (EET) is a critical form of microbial metabolism that enables respiration on a variety of inorganic substrates, including metal oxides. However, quantifying current generated by electroactive bacteria has been predominately limited to biofilms formed on electrodes. To address this, we developed a platform for quantifying EET flux from cell suspensions using aqueous dispersions of infrared plasmonic tin-doped indium oxide nanocrystals. Tracking the change in optical extinction during electron transfer enabled quantification of current generated by planktonic Shewanella oneidensis cultures. Using this method, we differentiated between starved and actively respiring cells, between cells of varying genotype, and between cells engineered to differentially express a key EET gene using an inducible genetic circuit. Overall, our results validate the utility of colloidally stable plasmonic metal oxide nanocrystals as quantitative biosensors in native biological environments and contribute to a fundamental understanding of planktonic S. oneidensis electrophysiology using simple in situ spectroscopy.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":127514,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":336297,"name":"Stephen L. Gibbs","orcid":"0000-0003-2533-0957","position":1,"is_corresponding":false},{"id":576357,"name":"Camila A. Saez Cabezas","orcid":"0000-0002-8734-0096","position":2,"is_corresponding":false},{"id":576791,"name":"Yongdan Wang","orcid":null,"position":3,"is_corresponding":false},{"id":467046,"name":"Allison Green","orcid":"0000-0002-7734-4202","position":4,"is_corresponding":false},{"id":336302,"name":"Delia J. Milliron","orcid":"0000-0002-8737-451X","position":5,"is_corresponding":false},{"id":282390,"name":"Benjamin K. Keitz","orcid":"0000-0003-3314-0053","position":6,"is_corresponding":false},{"id":282387,"name":"Austin J. Graham","orcid":"0000-0001-8924-181X","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-18T23:15:30.930746Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}