{"doi":"10.1101/2020.10.11.335315","title":"Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural products","abstract":"Abstract Bacterial hormones, such as the iconic gamma-butyrolactone A-factor, are essential signaling molecules that regulate diverse physiological processes, including specialized metabolism. These low molecular weight compounds are common in Streptomyces species and display species-specific structural differences. Recently, unusual gamma-butyrolactone natural products called salinipostins were isolated from the marine actinomycete genus Salinispora based on their anti-malarial properties. As the salinipostins possess a rare phosphotriester motif of unknown biosynthetic origin, we set out to explore its construction by the widely conserved 9-gene spt operon in Salinispora species. We show through a series of in vivo and in vitro studies that the spt gene cluster dually encodes the saliniphostins and newly identified A-factor-like gamma-butyrolactones (Sal-GBLs). Remarkably, homologous biosynthetic gene clusters are widely distributed amongst many actinomycete genera, including Streptomyces, suggesting the significance of this operon in bacteria.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":127452,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9517,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":576240,"name":"Takayoshi Awakawa","orcid":"0000-0003-2575-3175","position":1,"is_corresponding":false},{"id":576241,"name":"Yi‐Ling Du","orcid":"0000-0001-7072-4803","position":2,"is_corresponding":false},{"id":576242,"name":"Peter A. Jordan","orcid":"0000-0002-1773-3310","position":3,"is_corresponding":false},{"id":418607,"name":"Kaitlin E. Creamer","orcid":"0000-0002-0666-2107","position":4,"is_corresponding":false},{"id":262006,"name":"Paul R. Jensen","orcid":"0000-0003-2349-1888","position":5,"is_corresponding":false},{"id":261980,"name":"Roger G. Linington","orcid":"0000-0003-1818-4971","position":6,"is_corresponding":false},{"id":576243,"name":"Katherine S. Ryan","orcid":"0000-0003-1643-1940","position":7,"is_corresponding":false},{"id":261966,"name":"Bradley S. Moore","orcid":"0000-0002-4652-1253","position":8,"is_corresponding":false},{"id":576239,"name":"Yuta Kudo","orcid":"0000-0002-1586-2004","position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":null,"created_at":"2026-07-18T23:15:30.930746Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}