{"doi":"10.1101/2020.10.04.324525","title":"CXCL10 <sup>+</sup> peripheral activation niches couple preferred sites of Th1 entry with optimal APC encounter","abstract":"SUMMARY Correct positioning of T cells within infected tissues is critical for T cell activation and pathogen control. Upon tissue entry, effector T cells must efficiently locate antigen presenting cells (APC) for peripheral activation. We reveal that tissue entry and initial peripheral activation of Th1 effector T cells are tightly linked to perivascular positioning of chemokine-expressing APCs. Dermal inflammation induced tissue-wide de novo generation of discrete perivascular CXCL10 + cell clusters, enriched for CD11c + MHC-II + monocyte-derived dendritic cells. These chemokine clusters were ‘hot spots’ for both Th1 extravasation and activation in the inflamed skin. CXCR3-dependent Th1 localization to the cluster micro-environment prolonged T-APC interactions and boosted function. Both the frequency and range of these clusters were enhanced via a Th1-intrinsic, IFNγ-dependent positive feedback loop. Thus, the perivascular CXCL10 + clusters act as initial peripheral activation niches, optimizing controlled activation broadly throughout the tissue by coupling Th1 tissue entry with enhanced opportunities for Th1-APC encounter.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":127434,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9468,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":576206,"name":"Nilesh Patil","orcid":"0000-0002-3438-8571","position":1,"is_corresponding":false},{"id":576207,"name":"Seble G. Negatu","orcid":"0000-0002-6195-0312","position":2,"is_corresponding":false},{"id":362172,"name":"Alexander I. McGurk","orcid":"0000-0002-1033-9943","position":3,"is_corresponding":false},{"id":332444,"name":"Scott A. Leddon","orcid":"0000-0002-8226-6042","position":4,"is_corresponding":false},{"id":576719,"name":"Angela Hughson","orcid":null,"position":5,"is_corresponding":false},{"id":576208,"name":"Tristan D. McRae","orcid":"0000-0002-1823-1451","position":6,"is_corresponding":false},{"id":576209,"name":"Yu‐Rong Gao","orcid":"0000-0001-8363-6757","position":7,"is_corresponding":false},{"id":576210,"name":"Alexandra M. Livingstone","orcid":"0000-0003-4511-8363","position":8,"is_corresponding":false},{"id":576211,"name":"Joanna R. Groom","orcid":"0000-0001-5251-7835","position":9,"is_corresponding":false},{"id":256462,"name":"Andrew D. Luster","orcid":"0000-0001-9679-7912","position":10,"is_corresponding":false},{"id":326992,"name":"Deborah J. Fowell","orcid":"0000-0001-7093-4448","position":11,"is_corresponding":false},{"id":349408,"name":"Hen Prizant","orcid":null,"position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-18T23:15:30.930746Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}