{"doi":"10.1101/2020.10.01.322347","title":"Arabidopsis ACINUS is O-glycosylated and regulates transcription and alternative splicing of regulators of reproductive transitions","abstract":"Abstract O-GlcNAc modification plays important roles in metabolic regulation of cellular status. Two homologs of O-GlcNAc transferase, SECRET AGENT (SEC) and SPINDLY (SPY), which have O-GlcNAc and O-fucosyl transferase activities, respectively, are essential in Arabidopsis but have largely unknown cellular targets. Here we show that AtACINUS is O-GlcNAcylated and O-fucosylated and mediates regulation of transcription, alternative splicing (AS), and developmental transitions. Knocking-out both AtACINUS and its distant paralog AtPININ causes severe growth defects including dwarfism, delayed seed germination and flowering, and abscisic acid (ABA) hypersensitivity. Transcriptomic and protein-DNA/RNA interaction analyses demonstrate that AtACINUS represses transcription of the flowering repressor FLC and mediates AS of ABH1 and HAB1 , two negative regulators of ABA signaling. Proteomic analyses show AtACINUS’s O-GlcNAcylation, O-fucosylation, and association with splicing factors, chromatin remodelers, and transcriptional regulators. Some AtACINUS/AtPININ-dependent AS events are altered in the sec and spy mutants, demonstrating a function of O-glycosylation in regulating alternative RNA splicing.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":127437,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":576216,"name":"Zhiping Deng","orcid":"0000-0001-9663-3088","position":1,"is_corresponding":false},{"id":576217,"name":"Weimin Ni","orcid":"0000-0003-4481-2310","position":2,"is_corresponding":false},{"id":576725,"name":"Ruben Shretha","orcid":null,"position":3,"is_corresponding":false},{"id":576726,"name":"Dasha Savage","orcid":null,"position":4,"is_corresponding":false},{"id":576218,"name":"Thomas Hartwig","orcid":"0000-0002-2707-2771","position":5,"is_corresponding":false},{"id":576219,"name":"Sunita Patil","orcid":"0000-0001-8449-6821","position":6,"is_corresponding":false},{"id":293143,"name":"Su Hyun Hong","orcid":"0000-0001-9291-5803","position":7,"is_corresponding":false},{"id":275199,"name":"Juan A. Osés-Prieto","orcid":"0000-0003-4759-2341","position":8,"is_corresponding":false},{"id":576727,"name":"Kathy H. Li","orcid":null,"position":9,"is_corresponding":false},{"id":301843,"name":"Peter H. Quail","orcid":"0000-0001-6064-3460","position":10,"is_corresponding":false},{"id":256139,"name":"Alma L. Burlingame","orcid":"0000-0002-8403-7307","position":11,"is_corresponding":false},{"id":293144,"name":"Shou‐Ling Xu","orcid":"0000-0002-6741-9506","position":12,"is_corresponding":false},{"id":292355,"name":"Zhiyong Wang","orcid":"0000-0003-4602-3390","position":13,"is_corresponding":false},{"id":576215,"name":"Yang Bi","orcid":"0000-0003-1270-1747","position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-18T23:15:30.930746Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}