{"doi":"10.1101/2020.09.30.317214","title":"Implantable photonic neural probes for light-sheet fluorescence brain imaging","abstract":"ABSTRACT Significance Light-sheet fluorescence microscopy is a powerful technique for high-speed volumetric functional imaging. However, in typical light-sheet microscopes, the illumination and collection optics impose significant constraints upon the imaging of non-transparent brain tissues. Here, we demonstrate that these constraints can be surmounted using a new class of implantable photonic neural probes . Aim Mass manufacturable, silicon-based light-sheet photonic neural probes can generate planar patterned illumination at arbitrary depths in brain tissues without any additional micro-optic components. Approach We develop implantable photonic neural probes that generate light sheets in tissue. The probes were fabricated in a photonics foundry on 200 mm diameter silicon wafers. The light sheets were characterized in fluorescein and in free space. The probe-enabled imaging approach was tested in fixed and in vitro mouse brain tissues. Imaging tests were also performed using fluorescent beads suspended in agarose. Results The probes had 5 to 10 addressable sheets and average sheet thicknesses < 16 μm for propagation distances up to 300 μm in free space. Imaging areas were as large as ≈ 240 μm × 490 μm in brain tissue. Image contrast was enhanced relative to epifluorescence microscopy. Conclusions The neural probes can lead to new variants of light-sheet fluorescence microscopy for deep brain imaging and experiments in freely-moving animals.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":122313,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9484,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":563270,"name":"Fu‐Der Chen","orcid":"0000-0001-5482-2884","position":1,"is_corresponding":false},{"id":563271,"name":"Homeira Moradi‐Chameh","orcid":"0000-0002-8660-3595","position":2,"is_corresponding":false},{"id":563272,"name":"Xianshu Luo","orcid":"0000-0002-5531-5689","position":3,"is_corresponding":false},{"id":563273,"name":"Anton Fomenko","orcid":"0000-0003-4131-6784","position":4,"is_corresponding":false},{"id":563274,"name":"Prajay Shah","orcid":"0000-0002-6668-9301","position":5,"is_corresponding":false},{"id":564016,"name":"Thomas Lordello","orcid":null,"position":6,"is_corresponding":false},{"id":563275,"name":"Xinyu Liu","orcid":"0000-0002-4347-1633","position":7,"is_corresponding":false},{"id":564017,"name":"Ilan Felts Almog","orcid":null,"position":8,"is_corresponding":false},{"id":564018,"name":"John N. Straguzzi","orcid":null,"position":9,"is_corresponding":false},{"id":564019,"name":"Trevor M. Fowler","orcid":null,"position":10,"is_corresponding":false},{"id":563276,"name":"Youngho Jung","orcid":"0000-0001-8627-5168","position":11,"is_corresponding":false},{"id":563277,"name":"Ting Hu","orcid":"0000-0002-7134-1748","position":12,"is_corresponding":false},{"id":563278,"name":"Junho Jeong","orcid":"0000-0002-7179-4384","position":13,"is_corresponding":false},{"id":232900,"name":"Andrés M. Lozano","orcid":"0000-0001-8257-3694","position":14,"is_corresponding":false},{"id":563279,"name":"Patrick Lo","orcid":"0000-0002-5879-8364","position":15,"is_corresponding":false},{"id":460986,"name":"Taufik A. Valiante","orcid":"0000-0002-3443-3790","position":16,"is_corresponding":false},{"id":310889,"name":"Laurent Moreaux","orcid":"0000-0003-1276-5062","position":17,"is_corresponding":false},{"id":310895,"name":"Joyce K. S. Poon","orcid":"0000-0002-3691-4344","position":18,"is_corresponding":false},{"id":68801,"name":"M. L. Roukes","orcid":"0000-0002-2916-6026","position":19,"is_corresponding":false},{"id":310891,"name":"Wesley D. Sacher","orcid":"0000-0003-3306-4556","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-18T23:14:51.076430Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}