{"doi":"10.1101/2020.09.29.318477","title":"Chromosome-level genome assemblies of the malaria vectors <i>Anopheles coluzzii</i> and <i>Anopheles arabiensis</i>","abstract":"Abstract Background Anopheles coluzzii and An. arabiensis belong to the An. gambiae complex and are among the major malaria vectors in Sub-Saharan Africa. However, chromosome-level reference genome assemblies are still lacking for these medically important mosquito species. Findings In this study, we produced de novo chromosome-level genome assemblies for An. coluzzii and An. arabiensis using the long-read Oxford Nanopore sequencing technology and the Hi-C scaffolding approach. We obtained 273.4 Mbp and 256.8 Mbp of the total assemblies for An. coluzzii and An. arabiensis , respectively. Each assembly consists of three chromosome-scale scaffolds (X, 2, 3), complete mitochondrion, and unordered contigs identified as autosomal pericentromeric DNA, X pericentromeric DNA, and Y sequences. Comparison of these assemblies with the existing assemblies for these species demonstrated that we obtained improved reference-quality genomes. The new assemblies allowed us to identify genomiccoordinates for the breakpoint regions of fixed and polymorphic chromosomal inversions in An. coluzzii and An. arabiensis . Conclusion The new chromosome-level assemblies will facilitate functional and population genomic studies in An. coluzzii and An. arabiensis . The presented assembly pipeline will accelerate progress toward creating high-quality genome references for other disease vectors.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123026,"datarank":0.2559530596119301,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.014537372746815028,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.014537372746815028,"corpus_percentile":40.705500116036205,"corpus_rank":7666,"citation_count":4,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.8994,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":355306,"name":"Pavel Avdeyev","orcid":"0000-0002-7953-6259","position":1,"is_corresponding":false},{"id":489096,"name":"Jiangtao Liang","orcid":"0000-0002-8468-7457","position":2,"is_corresponding":false},{"id":262205,"name":"Atashi Sharma","orcid":"0000-0001-6299-7553","position":3,"is_corresponding":false},{"id":489097,"name":"Chujia Chen","orcid":"0000-0002-9196-1274","position":4,"is_corresponding":false},{"id":489098,"name":"Varvara Lukyanchikova","orcid":"0000-0002-1828-4721","position":5,"is_corresponding":false},{"id":355308,"name":"Nikita Alexeev","orcid":"0000-0003-3415-9565","position":6,"is_corresponding":false},{"id":262211,"name":"Zhijian Tu","orcid":"0000-0003-4227-3819","position":7,"is_corresponding":false},{"id":72985,"name":"Max A. Alekseyev","orcid":"0000-0002-5140-8095","position":8,"is_corresponding":false},{"id":50345,"name":"Igor V. Sharakhov","orcid":"0000-0003-0752-3747","position":9,"is_corresponding":false},{"id":565107,"name":"Anton Zamyatin","orcid":"0000-0001-5402-1796","position":0,"is_corresponding":true}],"reference_count":100,"raw_metadata":null,"created_at":"2026-07-18T23:14:55.385653Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}