{"doi":"10.1101/2020.09.25.313395","title":"High-throughput whole-brain mapping of rhesus monkey at micron resolution","abstract":"Abstract Whole-brain mesoscale mapping of primates has been hindered by large brain size and the relatively low throughput of available microscopy methods. Here, we present an integrative approach that combines primate-optimized tissue sectioning and clearing with ultrahigh-speed, large-scale, volumetric fluorescence microscopy, capable of completing whole-brain imaging of a rhesus monkey at 1 µm × 1 µm × 2.5 µm voxel resolution within 100 hours. A progressive strategy is developed for high-efficiency, long-range tracing of individual axonal fibers through the dataset of hundreds of terabytes, establishing a “Serial sectioning and clearing, 3-dimensional Microscopy, with semi-Automated Reconstruction and Tracing” (SMART) pipeline. This system supports effective connectome-scale mapping of large primates that reveals distinct features of thalamocortical projections of the rhesus monkey brain at the level of individual axonal fibers.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":124056,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.899,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":397823,"name":"Yan Shen","orcid":"0000-0002-7895-2963","position":1,"is_corresponding":false},{"id":569207,"name":"Lufeng Ding","orcid":null,"position":2,"is_corresponding":false},{"id":568244,"name":"Chao‐Yu Yang","orcid":"0000-0003-1864-9353","position":3,"is_corresponding":false},{"id":569208,"name":"Heng Tan","orcid":null,"position":4,"is_corresponding":false},{"id":437275,"name":"Hao Wang","orcid":"0000-0001-7958-3090","position":5,"is_corresponding":false},{"id":568245,"name":"Qingyuan Zhu","orcid":"0000-0001-5521-5023","position":6,"is_corresponding":false},{"id":568246,"name":"Rui Xu","orcid":"0000-0001-8526-3392","position":7,"is_corresponding":false},{"id":568247,"name":"Fengyi Wu","orcid":"0009-0005-7770-2363","position":8,"is_corresponding":false},{"id":467208,"name":"Cheng Xu","orcid":"0000-0002-3389-2215","position":9,"is_corresponding":false},{"id":568248,"name":"Qianwei Li","orcid":"0000-0002-5112-1449","position":10,"is_corresponding":false},{"id":568249,"name":"Peng Su","orcid":"0000-0002-8713-7274","position":11,"is_corresponding":false},{"id":286349,"name":"Li I. Zhang","orcid":"0000-0003-0275-8651","position":12,"is_corresponding":false},{"id":59097,"name":"Hong‐Wei Dong","orcid":"0000-0001-9972-3177","position":13,"is_corresponding":false},{"id":255653,"name":"Robert Desimone","orcid":"0000-0002-5938-4227","position":14,"is_corresponding":false},{"id":379401,"name":"Fuqiang Xu","orcid":"0000-0002-4382-9797","position":15,"is_corresponding":false},{"id":568250,"name":"Xintian Hu","orcid":"0000-0003-1216-4806","position":16,"is_corresponding":false},{"id":270837,"name":"Pak-Ming Lau","orcid":"0000-0001-9026-5501","position":17,"is_corresponding":false},{"id":255652,"name":"Guo‐Qiang Bi","orcid":"0000-0001-8735-3818","position":18,"is_corresponding":false},{"id":568243,"name":"Fang Xu","orcid":"0000-0003-1458-9875","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-18T23:15:07.789881Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}