{"doi":"10.1101/2020.09.24.295337","title":"Tissue Dynamics Spectroscopic Imaging: Functional Imaging of Heterogeneous Cancer Tissue","abstract":"Abstract Significance Tumor heterogeneity poses a challenge for the chemotherapeutic treatment of cancer. Tissue dynamics spectroscopy (TDS) captures dynamic contrast and can capture the response of living tissue to applied therapeutics, but the current analysis averages over the complicated spatial response of living biopsy samples. Aim To develop tissue dynamics spectroscopic imaging (TDSI) to map the heterogeneous spatial response of tumor tissue to anticancer drugs. Approach TDSI is applied to tumor spheroids grown from cell lines and to ex vivo living esophageal biopsy samples. Doppler fluctuation spectroscopy is performed on a voxel basis to extract spatial maps of biodynamic biomarkers. Functional images and bivariate spatial maps are produced using a bivariate color merge to represent the spatial distribution of pairs of signed drug-response biodynamic biomarkers. Results We have mapped the spatial variability of drug responses within biopsies and have tracked sample-to-sample variability. Sample heterogeneity observed in the biodynamic maps is associated with histological heterogeneity observed using inverted Selective-Plane Illumination Microscopy (iSPIM). Conclusion We have demonstrated the utility of TDSI as a functional imaging method to measure tumor heterogeneity and its potential for use in drug-response profiling.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":131103,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.944,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":526552,"name":"Bihe Hu","orcid":null,"position":1,"is_corresponding":false},{"id":584460,"name":"Guang Li","orcid":"0000-0002-2692-4004","position":2,"is_corresponding":false},{"id":226695,"name":"Sharon Fox","orcid":"0000-0002-0766-5393","position":3,"is_corresponding":false},{"id":289285,"name":"Shadia I. Jalal","orcid":"0000-0001-8938-7077","position":4,"is_corresponding":false},{"id":378928,"name":"John Turek","orcid":"0000-0003-4631-7356","position":5,"is_corresponding":false},{"id":449466,"name":"J. Quincy Brown","orcid":"0000-0002-8228-2667","position":6,"is_corresponding":false},{"id":378929,"name":"David D. Nolte","orcid":"0000-0002-4872-9357","position":7,"is_corresponding":false},{"id":584459,"name":"Zhe Li","orcid":"0009-0009-3725-2248","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-18T23:16:00.235845Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}