{"doi":"10.1101/2020.09.18.304410","title":"A global timing mechanism regulates cell-type specific wiring programs","abstract":"The assembly of neural circuits is dependent upon precise spatiotemporal expression of cell recognition molecules 1–6 . Factors controlling cell-type specificity have been identified 7–9 , but how timing is determined remains unknown. Here we describe the induction of a cascade of transcription factors by a steroid hormone (Ecdysone) in all fly visual system neurons spanning target recognition and synaptogenesis. We demonstrate through single cell sequencing that the Ecdysone pathway regulates the expression of a common set of targets required for synaptic maturation and cell-type specific targets enriched for cell surface proteins regulating wiring specificity. Transcription factors in the cascade regulate the expression of the same wiring genes in complex ways, including activation in one cell-type and repression in another. We show that disruption of the Ecdysone-pathway generates specific defects in dendritic and axonal processes and synaptic connectivity, with the order of transcription factor expression correlating with sequential steps in wiring. We also identify shared targets of a cell-type specific transcription factor and the Ecdysone pathway which regulate specificity. We propose neurons integrate a global temporal transcriptional module with cell-type specific transcription factors to generate different cell-type specific patterns of cell recognition molecules regulating wiring.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123014,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9512,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":344891,"name":"Ying Lin","orcid":"0000-0001-9336-1478","position":1,"is_corresponding":false},{"id":244617,"name":"Yerbol Z. Kurmangaliyev","orcid":"0000-0003-4829-2025","position":2,"is_corresponding":false},{"id":565084,"name":"Javier Valdés-Alemán","orcid":"0000-0002-1577-1057","position":3,"is_corresponding":false},{"id":565085,"name":"Samuel A. LoCascio","orcid":"0000-0002-6273-7302","position":4,"is_corresponding":false},{"id":565616,"name":"Parmis Mirshahidi","orcid":null,"position":5,"is_corresponding":false},{"id":565086,"name":"Brianna Parrington","orcid":"0000-0002-8516-7875","position":6,"is_corresponding":false},{"id":231545,"name":"S Lawrence Zipursky","orcid":"0000-0001-5630-7181","position":7,"is_corresponding":false},{"id":533251,"name":"Saumya Jain","orcid":"0000-0002-4083-3918","position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":null,"created_at":"2026-07-18T23:14:55.385653Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}