{"doi":"10.1101/2020.09.18.303909","title":"Cell type-selective secretome profiling in vivo","abstract":"ABSTRACT Secreted polypeptides are a fundamental biochemical axis of intercellular and endocrine communication. However, a global understanding of composition and dynamics of cellular secretomes in intact mammalian organisms has been lacking. Here, we introduce a proximity biotinylation strategy that enables labeling, detection, and enrichment of secreted polypeptides in a cell type-selective manner in mice. We generate a proteomic atlas of hepatocyte, myocyte, pericyte, and myeloid cell secretomes by direct purification of biotinylated secreted polypeptides from blood. Our secretome atlas validates known cell type-protein pairs, reveals secreted polypeptides that distinguish between cell types, and identifies new cellular sources for classical plasma proteins. Lastly, we uncover a dynamic and previously undescribed nutrient-dependent reprogramming of the hepatocyte secretome characterized by increased unconventional secretion of the cytosolic enzyme BHMT. This secretome profiling strategy enables dynamic and cell-type dissection of the plasma proteome and the secreted polypeptides that mediate intercellular signaling.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123016,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8888,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":218283,"name":"Nicholas M. Riley","orcid":"0000-0002-1536-2966","position":1,"is_corresponding":false},{"id":565087,"name":"Andrew C. Yang","orcid":"0000-0001-6445-734X","position":2,"is_corresponding":false},{"id":259633,"name":"Joon T. Kim","orcid":"0000-0002-6579-2278","position":3,"is_corresponding":false},{"id":259634,"name":"Stephanie M. Terrell","orcid":"0000-0001-7776-4621","position":4,"is_corresponding":false},{"id":259635,"name":"Veronica L. Li","orcid":"0000-0001-8888-3853","position":5,"is_corresponding":false},{"id":259636,"name":"Marta García-Contreras","orcid":"0000-0001-8014-7966","position":6,"is_corresponding":false},{"id":218284,"name":"Carolyn R. Bertozzi","orcid":"0000-0003-4482-2754","position":7,"is_corresponding":false},{"id":259637,"name":"Jonathan Z. Long","orcid":"0000-0003-2631-7463","position":8,"is_corresponding":false},{"id":259632,"name":"Wei Wei","orcid":"0000-0001-8110-0705","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-18T23:14:55.385653Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}