{"doi":"10.1101/2020.09.16.298463","title":"High-resolution Crystal Structures of Transient Intermediates in the Phytochrome Photocycle","abstract":"Abstract Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety of important physiological responses. They display a reversible photocycle between the resting (dark) Pr state and the light activated Pfr state, in which light signals are received and transduced as structural change through the entire protein to modulate the activity of the protein. It is unknown how the Pr-to-Pfr interconversion occurs as the structure of intermediates remain notoriously elusive. Here, we present short-lived crystal structures of the classical phytochrome from myxobacterium Stigmatella aurantiaca captured by an X-ray Free Electron Laser 5 ns and 33ms after light illumination of the Pr state. We observe large structural displacements of the covalently bound bilin chromophore, which trigger a bifurcated signaling pathway. The snapshots show with atomic precision how the signal progresses from the chromophore towards the output domains, explaining how plants, bacteria and fungi sense red light.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":121312,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9523,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":560599,"name":"Suraj Pandey","orcid":"0000-0002-7097-2185","position":1,"is_corresponding":false},{"id":482370,"name":"Juan C Sanchez","orcid":"0000-0002-1178-183X","position":2,"is_corresponding":false},{"id":560600,"name":"Moraima Noda","orcid":"0000-0002-7582-2127","position":3,"is_corresponding":false},{"id":560601,"name":"Ishwor Poudyal","orcid":"0000-0002-3498-9801","position":4,"is_corresponding":false},{"id":561259,"name":"Luis Aldama","orcid":null,"position":5,"is_corresponding":false},{"id":560602,"name":"Tek Narsingh Malla","orcid":"0000-0002-1387-0801","position":6,"is_corresponding":false},{"id":504225,"name":"Elin Claesson","orcid":"0000-0001-8232-7725","position":7,"is_corresponding":false},{"id":560603,"name":"Weixiao Yuan Wahlgren","orcid":"0000-0003-0413-165X","position":8,"is_corresponding":false},{"id":561260,"name":"Denisse Feliz","orcid":null,"position":9,"is_corresponding":false},{"id":560604,"name":"V. Šrajer","orcid":"0000-0003-0710-290X","position":10,"is_corresponding":false},{"id":560605,"name":"Michał Maj","orcid":"0000-0003-1567-9514","position":11,"is_corresponding":false},{"id":560606,"name":"Leticia Castillon","orcid":"0009-0007-2110-3825","position":12,"is_corresponding":false},{"id":352904,"name":"So Iwata","orcid":"0000-0003-1735-2937","position":13,"is_corresponding":false},{"id":352888,"name":"Eriko Nango","orcid":"0000-0001-9851-7355","position":14,"is_corresponding":false},{"id":352898,"name":"Rie Tanaka","orcid":"0000-0003-1062-4792","position":15,"is_corresponding":false},{"id":352897,"name":"Tomoyuki Tanaka","orcid":"0000-0002-9886-5947","position":16,"is_corresponding":false},{"id":560607,"name":"Fangjia Luo","orcid":"0000-0003-0631-6236","position":17,"is_corresponding":false},{"id":240880,"name":"Kensuke Tono","orcid":"0000-0003-1218-3759","position":18,"is_corresponding":false},{"id":240881,"name":"Shigeki Owada","orcid":"0000-0002-1451-7612","position":19,"is_corresponding":false},{"id":504228,"name":"Sebastian Westenhoff","orcid":"0000-0002-6961-8015","position":20,"is_corresponding":false},{"id":560608,"name":"Emina A. Stojković","orcid":"0000-0002-4971-5213","position":21,"is_corresponding":false},{"id":560609,"name":"Marius Schmidt","orcid":"0000-0002-0962-9468","position":22,"is_corresponding":false},{"id":560598,"name":"Melissa Carrillo","orcid":"0000-0002-0744-4486","position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-18T23:14:42.734024Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}