{"doi":"10.1101/2020.09.16.285841","title":"Disruption of Glial Ca <sup>2+</sup> Oscillations at the <i>Drosophila</i> Blood-Brain Barrier Predisposes to Seizure-Like Behavior","abstract":"Abstract Glia play key roles in regulating multiple aspects of neuronal development and function from invertebrates to humans. We recently found microdomain Ca 2+ signaling in Drosophila cortex glia and astrocytes regulate extracellular K + buffering and neurotransmitter uptake, respectively. Here we identify a role for ER store-operated Ca 2+ entry (SOCE) in perineurial glia (PG), a distinct population that contributes to the blood-brain barrier (BBB). PG show a diverse range of Ca 2+ oscillatory activity that varies based on their locale within the brain. Unlike cortex glia and astrocytes, PG Ca 2+ oscillations do not require extracellular Ca 2+ and are blocked by inhibition of SOCE or gap junctions. Disruption of these components triggers heat shock and mechanical-induced seizure-like episodes without effecting PG morphology or large molecule BBB permeability. These findings indicate SOCE-mediated Ca 2+ oscillations in PG increase the susceptibility of seizure-like episodes in Drosophila , providing an additional link between glial Ca 2+ signaling and neuronal activity.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":125370,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9435,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":571982,"name":"Lauren C. Clamon","orcid":null,"position":1,"is_corresponding":false},{"id":571315,"name":"Julia E. Manoim","orcid":"0000-0002-1581-9639","position":2,"is_corresponding":false},{"id":305495,"name":"Kiel G. Ormerod","orcid":"0000-0002-9739-8777","position":3,"is_corresponding":false},{"id":571316,"name":"Moshe Parnas","orcid":"0000-0001-9726-1511","position":4,"is_corresponding":false},{"id":305497,"name":"J. Troy Littleton","orcid":"0000-0001-5576-2887","position":5,"is_corresponding":false},{"id":571314,"name":"Shirley Weiss","orcid":"0000-0002-1006-349X","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-18T23:15:15.482227Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}