{"doi":"10.1101/2020.09.12.294066","title":"SARS-CoV-2 infection severity is linked to superior humoral immunity against the spike","abstract":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently causing a global pandemic. The antigen specificity and kinetics of the antibody response mounted against this novel virus are not understood in detail. Here, we report that subjects with a more severe SARS-CoV-2 infection exhibit a larger antibody response against the spike and nucleocapsid protein and epitope spreading to subdominant viral antigens, such as open reading frame 8 and non-structural proteins. Subjects with a greater antibody response mounted a larger memory B cell response against the spike, but not the nucleocapsid protein. Additionally, we revealed that antibodies against the spike are still capable of binding the D614G spike mutant and cross-react with the SARS-CoV-1 receptor binding domain. Together, this study reveals that subjects with a more severe SARS-CoV-2 infection exhibit a greater overall antibody response to the spike and nucleocapsid protein and a larger memory B cell response against the spike.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":119205,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9643,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":266560,"name":"Olivia Stovicek","orcid":"0000-0001-6413-855X","position":1,"is_corresponding":false},{"id":553707,"name":"Jiaolong Wang","orcid":"0000-0002-4357-3226","position":2,"is_corresponding":false},{"id":266563,"name":"Siriruk Changrob","orcid":"0000-0002-1826-4333","position":3,"is_corresponding":false},{"id":284391,"name":"Lei Li","orcid":"0000-0002-8828-5199","position":4,"is_corresponding":false},{"id":107104,"name":"Peter Halfmann","orcid":"0000-0002-1648-1625","position":5,"is_corresponding":false},{"id":269129,"name":"Nai‐Ying Zheng","orcid":null,"position":6,"is_corresponding":false},{"id":266564,"name":"Henry A. 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