{"doi":"10.1101/2020.09.08.286930","title":"Reconstitution of prospermatogonial specification <i>in vitro</i> from human induced pluripotent stem cells","abstract":"ABSTRACT Establishment of spermatogonia throughout the fetal and postnatal period is essential for production of spermatozoa and male fertility. Here, we established a protocol for in vitro reconstitution of human prospermatogonial specification whereby human primordial germ cell (PGC)-like cells (hPGCLCs) differentiated from human induced pluripotent stem cells were further induced into M-prospermatogonia-like cells (MLCs) and T1 prospermatogonia-like cells (T1LCs) using long-term cultured xenogeneic reconstituted testes. Single cell RNA-sequencing was used to delineate the lineage trajectory leading to T1LCs, which closely resemble human T1-prospermatogonia in vivo and exhibited gene expression related to spermatogenesis and diminished proliferation, a hallmark of quiescent T1 prospermatogonia. Notably, this system enabled us to visualize the dynamic and stage-specific regulation of transposable elements during human prospermatogonial specification. Together, our findings pave the way for understanding and reconstructing human male germline development in vitro .","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":130951,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9503,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":258981,"name":"Shinnosuke Suzuki","orcid":"0000-0003-0241-1455","position":1,"is_corresponding":false},{"id":258982,"name":"Yasunari Seita","orcid":"0000-0002-5020-1594","position":2,"is_corresponding":false},{"id":258983,"name":"Jumpei Ito","orcid":"0000-0003-0440-8321","position":3,"is_corresponding":false},{"id":258984,"name":"Yuka Sakata","orcid":"0000-0003-4254-2417","position":4,"is_corresponding":false},{"id":258985,"name":"Hirofumi Aso","orcid":"0000-0002-2826-3836","position":5,"is_corresponding":false},{"id":37911,"name":"Kei Sato","orcid":"0000-0003-4431-1380","position":6,"is_corresponding":false},{"id":258986,"name":"Brian P. Hermann","orcid":"0000-0002-0503-3616","position":7,"is_corresponding":false},{"id":258987,"name":"Kotaro Sasaki","orcid":"0000-0002-5604-2651","position":8,"is_corresponding":false},{"id":258980,"name":"Young Sun Hwang","orcid":"0000-0002-6443-9579","position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-18T23:16:00.235845Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}