{"doi":"10.1101/2020.08.29.272369","title":"Proximity labeling at non-centrosomal microtubule-organizing centers reveals VAB-10B and WDR-62 as distinct microtubule regulators","abstract":"Summary Reorganization of microtubules from the centrosome to non-centrosomal subcellular sites is central to cell differentiation. To identify components of non-centrosomal microtubule organizing centers in differentiated cells of a living organism, we developed the biotin ligase-based proximity labeling approach TurboID for use in C. elegans . We identified proteins proximal to the non-centrosomal microtubule minus end protein PTRN-1/Patronin at the apical membrane of epithelial cells, focusing on two conserved proteins: spectraplakin protein VAB-10B and WDR-62, a protein we identify as homologous to vertebrate primary microcephaly disease gene WDR62. We found that WDR-62 and VAB-10B independently regulate the growth and localization of non-centrosomal microtubules and the apical targeting of microtubule minus end proteins. This regulation occurs downstream of cell polarity and in conjunction with actin. Our data suggest a division of labor where microtubule growth and anchoring are regulated by distinct complexes and uncover novel functions of spectraplakins and WDR62 family proteins.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":121718,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.954,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":552756,"name":"Tess C. Branon","orcid":"0000-0002-6520-6419","position":1,"is_corresponding":false},{"id":562451,"name":"Lauren E. Cote","orcid":null,"position":2,"is_corresponding":false},{"id":561776,"name":"Alexandros Papagiannakis","orcid":"0000-0002-6363-804X","position":3,"is_corresponding":false},{"id":314616,"name":"Xing Liang","orcid":"0000-0002-8298-1214","position":4,"is_corresponding":false},{"id":561777,"name":"Melissa A. Pickett","orcid":"0000-0001-8364-4757","position":5,"is_corresponding":false},{"id":314620,"name":"Kang Shen","orcid":"0000-0003-4059-8249","position":6,"is_corresponding":false},{"id":495528,"name":"Christine Jacobs‐Wagner","orcid":"0000-0003-0980-5334","position":7,"is_corresponding":false},{"id":242245,"name":"Alice Y. Ting","orcid":"0000-0002-8277-5226","position":8,"is_corresponding":false},{"id":308279,"name":"Jessica L. Feldman","orcid":"0000-0002-5210-5045","position":9,"is_corresponding":false},{"id":562450,"name":"Ariana D. Sanchez","orcid":null,"position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-18T23:14:46.979435Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}