{"doi":"10.1101/2020.08.24.264531","title":"Methods for detecting PER2::LUCIFERASE bioluminescence rhythms in freely moving mice","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Circadian rhythms are driven by daily oscillations of gene expression. An important tool for studying cellular and tissue rhythms is the use of a gene reporter, such as bioluminescence from the reporter gene luciferase controlled by a rhythmically expressed gene of interest. Here we describe methods that allow measurement of bioluminescence from a freely-moving mouse housed in a standard cage. Using a LumiCycle\n                  <jats:italic>In Vivo</jats:italic>\n                  (Actimetrics), we determined conditions that allow detection of circadian rhythms of bioluminescence from the PER2 reporter, PER2::LUC, in freely behaving mice. We tested delivery of D-luciferin via a subcutaneous minipump and in the drinking water. Further, we demonstrate that a synthetic luciferase substrate, CycLuc1, can support circadian rhythms of bioluminescence, even when delivered at a lower concentration than D-luciferin. We share our analysis scripts and suggestions for further improvements in this method. This approach will be straightforward to apply to mice with tissue-specific reporters, allowing insights into responses of specific peripheral clocks to perturbations such as environmental or pharmacological manipulations.\n                </jats:p>","journal":null,"year":null,"id":604169,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":475070,"name":"Wanqi Wang","orcid":"0000-0001-7424-9165","position":1,"is_corresponding":false},{"id":579398,"name":"Ivana William","orcid":"0000-0002-9040-2526","position":2,"is_corresponding":false},{"id":446794,"name":"Selma Tir","orcid":"0000-0001-5469-4544","position":3,"is_corresponding":false},{"id":1550049,"name":"I. Mohammad","orcid":"0000-0002-4353-5633","position":4,"is_corresponding":false},{"id":1550050,"name":"R. Javed","orcid":null,"position":5,"is_corresponding":false},{"id":126405,"name":"S. Smith","orcid":null,"position":6,"is_corresponding":false},{"id":94556,"name":"Y. Cui","orcid":null,"position":7,"is_corresponding":false},{"id":425896,"name":"Ciearra B. Smith","orcid":"0000-0003-2999-3387","position":8,"is_corresponding":false},{"id":197216,"name":"Vincent van der Vinne","orcid":"0000-0003-3926-5041","position":9,"is_corresponding":false},{"id":1550051,"name":"P.C. Molyneux","orcid":null,"position":10,"is_corresponding":false},{"id":139348,"name":"Stephen C. Miller","orcid":"0000-0001-7154-7757","position":11,"is_corresponding":false},{"id":305965,"name":"David R. Weaver","orcid":"0000-0001-7941-6719","position":12,"is_corresponding":false},{"id":446795,"name":"Tanya Leise","orcid":"0000-0002-7458-7604","position":13,"is_corresponding":false},{"id":426098,"name":"Mary E. Harrington","orcid":"0000-0003-2266-6455","position":14,"is_corresponding":false},{"id":750077,"name":"Blanca Martin-Burgos","orcid":"0000-0002-1388-9123","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Methods for detecting PER2::LUCIFERASE bioluminescence rhythms in freely moving mice","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Circadian rhythms are driven by daily oscillations of gene expression. An important tool for studying cellular and tissue rhythms is the use of a gene reporter, such as bioluminescence from the reporter gene luciferase controlled by a rhythmically expressed gene of interest. Here we describe methods that allow measurement of bioluminescence from a freely-moving mouse housed in a standard cage. Using a LumiCycle\n                  <jats:italic>In Vivo</jats:italic>\n                  (Actimetrics), we determined conditions that allow detection of circadian rhythms of bioluminescence from the PER2 reporter, PER2::LUC, in freely behaving mice. We tested delivery of D-luciferin via a subcutaneous minipump and in the drinking water. Further, we demonstrate that a synthetic luciferase substrate, CycLuc1, can support circadian rhythms of bioluminescence, even when delivered at a lower concentration than D-luciferin. We share our analysis scripts and suggestions for further improvements in this method. This approach will be straightforward to apply to mice with tissue-specific reporters, allowing insights into responses of specific peripheral clocks to perturbations such as environmental or pharmacological manipulations.\n                </jats:p>","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":"https://openalex.org/W3081169494","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2021,"count":2},{"year":2022,"count":2}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2020/08/24/2020.08.24.264531.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2020/08/24/2020.08.24.264531.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2020.08.24.264531","host_type":"publisher"},{"url":"https://doi.org/10.1101/2020.08.24.264531","host_type":"repository"},{"url":"https://scholarworks.smith.edu/psy_facpubs/87","host_type":"repository"}],"fields_of_study":["Circadian rhythm and melatonin","Light effects on plants","Neurobiology and Insect Physiology Research"],"mesh_terms":[],"keywords":["PER2","Bioluminescence","Luciferase","Circadian rhythm","Bioluminescence imaging","Luciferin","Reporter gene","Rhythm","Biology","CLOCK","Cell biology","Gene","Neuroscience","Circadian clock","Gene expression","Biochemistry","Internal medicine","Medicine","Transfection"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T23:28:09.708309Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}