{"doi":"10.1101/2020.08.24.264069","title":"Single cell BCR and transcriptome analysis after respiratory virus infection reveals spatiotemporal dynamics of antigen-specific B cell responses","abstract":"SUMMARY B cell responses are a critical component of anti-viral immunity. However, a comprehensive picture of antigen-specific B cell responses, differentiation, clonal proliferation and dynamics in different organs after infection is lacking. Here, we combined single-cell RNA sequencing with single-cell B cell receptor (BCR) characterization of antigen-specific cells in the draining lymph nodes, spleen and lungs after influenza infection. We identify several novel B cell subpopulations forming after infection and find organ-specific differences that persist over the course of the response. We discover important transcriptional differences between memory cells in lungs and lymphoid organs and describe organ-restricted clonal expansion. Strikingly, by combining BCR mutational analysis, monoclonal antibody expression and affinity measurements we find no differences between germinal center (GC)-derived memory and plasmacells, at odds with an affinity-based selection model. By linking antigen-recognition with transcriptional programming, clonal-proliferation and differentiation, these finding provide important advances in our understanding of antiviral B cell immunity.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123973,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9473,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":568154,"name":"Jayalal K. Jayanthan","orcid":null,"position":1,"is_corresponding":false},{"id":567652,"name":"И. В. Смирнов","orcid":"0000-0002-1341-825X","position":2,"is_corresponding":false},{"id":227453,"name":"Jonathan L. Robinson","orcid":"0000-0001-8567-5960","position":3,"is_corresponding":false},{"id":568155,"name":"Hannes Axelsson","orcid":null,"position":4,"is_corresponding":false},{"id":568156,"name":"Sravya Sowdamini Nakka","orcid":null,"position":5,"is_corresponding":false},{"id":567653,"name":"Aikaterini Emmanouilidi","orcid":"0000-0001-9431-6900","position":6,"is_corresponding":false},{"id":85024,"name":"Paulo Czarnewski","orcid":"0000-0001-8150-4021","position":7,"is_corresponding":false},{"id":567654,"name":"William T. Yewdell","orcid":"0000-0002-5712-1896","position":8,"is_corresponding":false},{"id":567655,"name":"Cristina Lebrero‐Fernández","orcid":"0000-0002-9024-1240","position":9,"is_corresponding":false},{"id":481149,"name":"Valentina Bernasconi","orcid":"0000-0001-8357-6838","position":10,"is_corresponding":false},{"id":568157,"name":"Ali M. Harandi","orcid":null,"position":11,"is_corresponding":false},{"id":378759,"name":"Nils Lycke","orcid":"0000-0003-1155-4861","position":12,"is_corresponding":false},{"id":251348,"name":"Nicholas Borcherding","orcid":"0000-0003-1427-6342","position":13,"is_corresponding":false},{"id":17894,"name":"Jonathan W. Yewdell","orcid":"0000-0002-3826-1906","position":14,"is_corresponding":false},{"id":567656,"name":"Victor Greiff","orcid":"0000-0003-2622-5032","position":15,"is_corresponding":false},{"id":567657,"name":"Mats Bemark","orcid":"0000-0001-7416-9819","position":16,"is_corresponding":false},{"id":325928,"name":"Davide Angeletti","orcid":"0000-0002-5256-1972","position":17,"is_corresponding":false},{"id":567651,"name":"Nimitha R. Mathew","orcid":"0000-0003-3942-695X","position":0,"is_corresponding":true}],"reference_count":77,"raw_metadata":null,"created_at":"2026-07-18T23:15:03.403566Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}