{"doi":"10.1101/2020.08.10.243329","title":"Durable Control of HIV-1 Using a <i>Staphylococcus aureus</i> Cas9-Expressing Lentivirus Co-Targeting Viral Latency and Host Susceptibility","abstract":"ABSTRACT CRISPR/Cas9 gene editing has the potential to revolutionize the clinical management of HIV-1 infection, and may eliminate the need for antiretroviral therapy (ART). Current gene therapies attempt to either excise HIV-1 provirus or target HIV-1 entry receptors to prevent infection of new cells. Using a viral dynamic model, we determined that combining these two interventions, in the presence or absence of ART, significantly lowers the gene editing efficacy thresholds required to achieve an HIV-1 cure. To implement this dual-targeting approach, we engineered a single lentiviral vector that simultaneously targets multiple highly-conserved regions of the provirus and the host CXCR4 coreceptor, and developed a novel coculture system enabling real-time monitoring of latent infection, viral reactivation, and infection of new target cells. Simultaneous dual-targeting depleted HIV-1-infected cells with significantly greater potency than vectors targeting either virus or host independently, highlighting its potential as an HIV-1 cure strategy.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":128364,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9499,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":578707,"name":"Nishith R. Reddy","orcid":"0000-0001-5912-6925","position":1,"is_corresponding":false},{"id":290820,"name":"Kyle A. Raymond","orcid":"0000-0001-8670-2852","position":2,"is_corresponding":false},{"id":537094,"name":"Mohamed S. Bouzidi","orcid":null,"position":3,"is_corresponding":false},{"id":578708,"name":"Shivani Desai","orcid":"0000-0001-6069-7354","position":4,"is_corresponding":false},{"id":86810,"name":"Zain Y. Dossani","orcid":"0000-0001-9264-6340","position":5,"is_corresponding":false},{"id":536381,"name":"Hannah S. Sperber","orcid":"0000-0002-9638-6555","position":6,"is_corresponding":false},{"id":103725,"name":"James Theiler","orcid":"0000-0003-1888-3684","position":7,"is_corresponding":false},{"id":24197,"name":"Bette Korber","orcid":"0000-0002-2026-5757","position":8,"is_corresponding":false},{"id":24132,"name":"Satish K. Pillai","orcid":"0000-0001-8770-7014","position":9,"is_corresponding":false},{"id":578706,"name":"Leonard Chávez","orcid":"0000-0003-3226-0897","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:15:38.879956Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}