{"doi":"10.1101/2020.08.03.234963","title":"A <i>Legionella</i> effector ADP-ribosyltransferase inactivates glutamate dehydrogenase","abstract":"Abstract ADP-ribosyltransferases (ARTs) are a widespread superfamily of enzymes frequently employed in pathogenic strategies of bacteria. Legionella pneumophila , the causative agent of Legionnaire’s disease, has acquired over 330 translocated effectors that showcase remarkable biochemical and structural diversity. Here we took a bioinformatic approach to search the Legionella effector repertoire for additional divergent members of the ART superfamily and identified an ART domain in Lpg0181. We show that L . pneumophila Lpg0181 targets a specific class of 120-kDa NAD+-dependent glutamate dehydrogenase (GDH) enzymes found in fungi and protists, including many natural hosts of Legionella . Lpg0181 targets a conserved arginine residue in the NAD+ -binding pocket of GDH, thereby blocking oxidative deamination of glutamate. While intracellular pathogens employ diverse virulence mechanisms to overcome host-limited nutrient availability, Lpg0181 is––to the best of our knowledge––the first example of a Legionella effector which directly targets a host metabolic enzyme.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":130632,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.955,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":583373,"name":"Adam Osinski","orcid":"0000-0003-0010-0733","position":1,"is_corresponding":false},{"id":583374,"name":"Marcin Gradowski","orcid":"0000-0001-7695-5434","position":2,"is_corresponding":false},{"id":488373,"name":"Kelly A. Servage","orcid":"0000-0001-7183-2865","position":3,"is_corresponding":false},{"id":393209,"name":"Krzysztof Pawłowski","orcid":"0000-0002-5367-0935","position":4,"is_corresponding":false},{"id":362003,"name":"Vincent S. Tagliabracci","orcid":"0000-0002-9735-4678","position":5,"is_corresponding":false},{"id":583372,"name":"Miles H. Black","orcid":"0000-0002-2864-1710","position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-18T23:15:56.698770Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}