{"doi":"10.1101/2020.08.02.233536","title":"Cross-neutralization of a SARS-CoV-2 antibody to a functionally conserved site is mediated by avidity","abstract":"Most antibodies isolated from COVID-19 patients are specific to SARS-CoV-2. COVA1-16 is a relatively rare antibody that also cross-neutralizes SARS-CoV. Here we determined a crystal structure of COVA1-16 Fab with the SARS-CoV-2 RBD, and a negative-stain EM reconstruction with the spike glycoprotein trimer, to elucidate the structural basis of its cross-reactivity. COVA1-16 binds a highly conserved epitope on the SARS-CoV-2 RBD, mainly through a long CDR H3, and competes with ACE2 binding due to steric hindrance rather than epitope overlap. COVA1-16 binds to a flexible up conformation of the RBD on the spike and relies on antibody avidity for neutralization. These findings, along with structural and functional rationale for the epitope conservation, provide a blueprint for development of more universal SARS-like coronavirus vaccines and therapies.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":118769,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":42,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9547,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":105424,"name":"Nicholas C. Wu","orcid":"0000-0002-9078-6697","position":1,"is_corresponding":false},{"id":105423,"name":"Meng Yuan","orcid":"0000-0001-9754-4503","position":2,"is_corresponding":false},{"id":230740,"name":"Sandhya Bangaru","orcid":"0000-0001-7994-4693","position":3,"is_corresponding":false},{"id":106545,"name":"Jonathan L. Torres","orcid":"0000-0003-0137-8497","position":4,"is_corresponding":false},{"id":240714,"name":"Tom G. Caniels","orcid":"0000-0001-9580-157X","position":5,"is_corresponding":false},{"id":240715,"name":"Jelle van Schooten","orcid":"0000-0002-5202-355X","position":6,"is_corresponding":false},{"id":105425,"name":"Xueyong Zhu","orcid":"0000-0002-6021-3740","position":7,"is_corresponding":false},{"id":105426,"name":"Chang‐Chun D. Lee","orcid":"0000-0002-5470-2484","position":8,"is_corresponding":false},{"id":240716,"name":"Philip J. M. Brouwer","orcid":"0000-0002-2902-7739","position":9,"is_corresponding":false},{"id":240717,"name":"Marit J. van Gils","orcid":"0000-0003-3422-8161","position":10,"is_corresponding":false},{"id":240718,"name":"Rogier W. Sanders","orcid":"0000-0002-2324-8573","position":11,"is_corresponding":false},{"id":230082,"name":"Andrew B. Ward","orcid":"0000-0001-7153-3769","position":12,"is_corresponding":false},{"id":105430,"name":"Ian A. Wilson","orcid":"0000-0002-6469-2419","position":13,"is_corresponding":false},{"id":108970,"name":"Hejun Liu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-18T23:14:03.409507Z","pmid":"32793906","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}