{"doi":"10.1101/2020.08.02.233056","title":"Efficient population modification gene-drive rescue system in the malaria mosquito <i>Anopheles stephensi</i>","abstract":"ABSTRACT The development of Cas9/gRNA-mediated gene-drive systems has bolstered the advancement of genetic technologies for controlling vector-borne pathogen transmission. These include population suppression approaches, genetic analogs of insecticidal techniques that reduce the number of vector insects, and population modification (replacement/alteration) approaches, which interfere with competence to transmit pathogens. We developed a recoded gene-drive rescue system for population modification in the malaria vector, Anopheles stephensi , that relieves the load in females caused by integration of the drive into the kynurenine hydroxylase gene by rescuing its function. Non-functional resistant alleles are eliminated via a dominantly-acting maternal effect combined with slower-acting standard negative selection, and a functional resistant allele does not prevent drive invasion. Small cage trials show that single releases of gene-drive males robustly result in efficient population modification with ≥95% of mosquitoes carrying the drive within 5-11 generations over a range of initial release ratios.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":121690,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9514,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":245841,"name":"Valentino M. Gantz","orcid":"0000-0003-2453-0711","position":1,"is_corresponding":false},{"id":245842,"name":"Nijole Jasinskiene","orcid":"0000-0002-0862-0349","position":2,"is_corresponding":false},{"id":245843,"name":"Hsu-Feng Lee","orcid":"0000-0001-5223-742X","position":3,"is_corresponding":false},{"id":245844,"name":"Kristy Hwang","orcid":"0000-0003-4581-6698","position":4,"is_corresponding":false},{"id":245846,"name":"Emily A. Bulger","orcid":"0000-0003-2967-1994","position":5,"is_corresponding":false},{"id":245847,"name":"Arunachalam Ramaiah","orcid":"0000-0003-3573-6141","position":6,"is_corresponding":false},{"id":245848,"name":"Jared B. Bennett","orcid":"0000-0003-4718-257X","position":7,"is_corresponding":false},{"id":245845,"name":"Gerard Terradas","orcid":"0000-0003-4285-4007","position":8,"is_corresponding":false},{"id":245849,"name":"J. J. Emerson","orcid":"0000-0001-9474-0891","position":9,"is_corresponding":false},{"id":245850,"name":"John M. Marshall","orcid":"0000-0003-0603-7341","position":10,"is_corresponding":false},{"id":245851,"name":"Ethan Bier","orcid":"0000-0002-2892-3005","position":11,"is_corresponding":false},{"id":245852,"name":"Anthony A. James","orcid":"0000-0001-5577-3308","position":12,"is_corresponding":false},{"id":245840,"name":"Adriana Adolfi","orcid":"0000-0003-0573-5076","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:14:46.979435Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}