{"doi":"10.1101/2020.07.16.206276","title":"Spelling changes and fluorescent tagging with prime editing vectors for plants","abstract":"Abstract Prime editing (PE) is a recent adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase (RT) fusion and a guide RNA (pegRNA) amended with template and primer binding site (PBS) sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of PE in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for PE in dicots and monocots, their validation in Nicotiana benthamiana , rice and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":120322,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9534,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":557449,"name":"Hilal Betül Kaya","orcid":"0000-0002-2543-7212","position":1,"is_corresponding":false},{"id":557450,"name":"Ning Zhang","orcid":"0000-0003-2775-1755","position":2,"is_corresponding":false},{"id":557451,"name":"Rhitu Rai","orcid":"0000-0002-4205-4336","position":3,"is_corresponding":false},{"id":557452,"name":"Matthew R. Willmann","orcid":"0000-0002-4146-8158","position":4,"is_corresponding":false},{"id":557453,"name":"Sara C. D. Carpenter","orcid":"0000-0002-3485-4042","position":5,"is_corresponding":false},{"id":269787,"name":"Andrew C. Read","orcid":"0000-0003-1789-6571","position":6,"is_corresponding":false},{"id":557454,"name":"Federico Martin","orcid":"0000-0002-7491-4468","position":7,"is_corresponding":false},{"id":279300,"name":"Zhangjun Fei","orcid":"0000-0001-9684-1450","position":8,"is_corresponding":false},{"id":557455,"name":"Jan E. Leach","orcid":"0000-0001-7252-4397","position":9,"is_corresponding":false},{"id":557456,"name":"Gregory B. Martin","orcid":"0000-0003-0044-6830","position":10,"is_corresponding":false},{"id":269788,"name":"Adam J. Bogdanove","orcid":"0000-0003-1683-4117","position":11,"is_corresponding":false},{"id":264404,"name":"Li Wang","orcid":"0000-0003-3722-5986","position":0,"is_corresponding":true}],"reference_count":10,"raw_metadata":null,"created_at":"2026-07-18T23:14:33.640077Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}