{"doi":"10.1101/2020.07.09.196386","title":"Replication-competent vesicular stomatitis virus vaccine vector protects against SARS-CoV-2-mediated pathogenesis","abstract":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused millions of human infections and hundreds of thousands of deaths. Accordingly, an effective vaccine is of critical importance in mitigating coronavirus induced disease 2019 (COVID-19) and curtailing the pandemic. We developed a replication-competent vesicular stomatitis virus (VSV)-based vaccine by introducing a modified form of the SARS-CoV-2 spike gene in place of the native glycoprotein gene (VSV-eGFP-SARS-CoV-2). Immunization of mice with VSV-eGFP-SARS-CoV-2 elicits high titers of antibodies that neutralize SARS-CoV-2 infection and target the receptor binding domain that engages human angiotensin converting enzyme-2 (ACE2). Upon challenge with a human isolate of SARS-CoV-2, mice expressing human ACE2 and immunized with VSV-eGFP-SARS-CoV-2 show profoundly reduced viral infection and inflammation in the lung indicating protection against pneumonia. Finally, passive transfer of sera from VSV-eGFP-SARS-CoV-2-immunized animals protects naïve mice from SARS-CoV-2 challenge. These data support development of VSV-eGFP-SARS-CoV-2 as an attenuated, replication-competent vaccine against SARS-CoV-2.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":120318,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9507,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":106713,"name":"Paul W. Rothlauf","orcid":"0000-0002-0941-4467","position":1,"is_corresponding":false},{"id":106500,"name":"Rita E. Chen","orcid":"0000-0002-5100-7750","position":2,"is_corresponding":false},{"id":106513,"name":"Natasha M. Kafai","orcid":"0000-0002-3115-3400","position":3,"is_corresponding":false},{"id":106515,"name":"Julie M. Fox","orcid":"0000-0003-0567-738X","position":4,"is_corresponding":false},{"id":106516,"name":"Swathi Shrihari","orcid":"0000-0002-8188-6936","position":5,"is_corresponding":false},{"id":106524,"name":"Broc T. McCune","orcid":"0000-0002-8342-5316","position":6,"is_corresponding":false},{"id":246333,"name":"Ian B. Harvey","orcid":"0000-0002-5444-8287","position":7,"is_corresponding":false},{"id":246332,"name":"Brittany Smith","orcid":"0009-0004-2057-9985","position":8,"is_corresponding":false},{"id":106525,"name":"Shamus P. Keeler","orcid":"0000-0002-1301-0852","position":9,"is_corresponding":false},{"id":226293,"name":"Louis-Marie Bloyet","orcid":"0000-0002-5648-3190","position":10,"is_corresponding":false},{"id":106514,"name":"Emma S. Winkler","orcid":"0000-0001-8510-1385","position":11,"is_corresponding":false},{"id":106526,"name":"Michael J. Holtzman","orcid":"0000-0001-8750-3716","position":12,"is_corresponding":false},{"id":225534,"name":"Daved H. Fremont","orcid":"0000-0002-8544-2689","position":13,"is_corresponding":false},{"id":103752,"name":"Sean P. J. Whelan","orcid":"0000-0003-1564-8590","position":14,"is_corresponding":false},{"id":106529,"name":"Michael Diamond","orcid":"0000-0002-8791-3165","position":15,"is_corresponding":false},{"id":106498,"name":"James Brett Case","orcid":"0000-0001-7331-5511","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:14:33.640077Z","pmid":"32676597","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}