{"doi":"10.1101/2020.07.06.189670","title":"Wide-Field Calcium Imaging of Dynamic Cortical Networks During Locomotion","abstract":"ABSTRACT Motor behavior results in widespread activation of the cerebral cortex. Therefore, fully understanding the cerebral cortex’s role in motor behavior requires a mesoscopic level description of the engaged cortical regions and their functional interactions. Mesoscopic imaging of Ca 2+ fluorescence through transparent polymer skulls implanted on transgenic Thy1-GCaMP6f mice reveals widespread activation of the cerebral cortex during locomotion, including not only in primary motor and somatosensory regions but also in secondary motor, retrosplenial, and visual cortices. Using the time series of Ca 2+ fluorescence from 28 regions (nodes) we found to be consistent across mice, we examined the changes in functional connectivity from rest to locomotion. At the initiation of locomotion, the most anterior nodes of the secondary motor cortex increase in correlation with other nodes, while other nodes decrease in correlation. Eigenvector centrality confirms these changes in functional connectivity. Directed Granger causality analysis reveals an increase in causal influence of anterior regions of secondary motor cortex on other dorsal cortical regions at the onset of locomotion. These results highlight the global changes in correlation, centrality, and causality occurring throughout the cerebral cortex between rest and locomotion and suggest that the premotor areas play an important role in organizing these changes.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":120021,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9481,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":556330,"name":"Justin D. Aronson","orcid":"0000-0001-6573-3000","position":1,"is_corresponding":false},{"id":556862,"name":"Laurentiu S. Popa","orcid":null,"position":2,"is_corresponding":false},{"id":556331,"name":"Russell E. Carter","orcid":"0000-0003-2163-673X","position":3,"is_corresponding":false},{"id":556332,"name":"Kathryn D. Feller","orcid":"0000-0003-4730-2105","position":4,"is_corresponding":false},{"id":556863,"name":"William M. Chiesl","orcid":null,"position":5,"is_corresponding":false},{"id":556864,"name":"Morgan L Gerhart","orcid":null,"position":6,"is_corresponding":false},{"id":476928,"name":"Aditya C. Shekhar","orcid":"0000-0002-2710-2854","position":7,"is_corresponding":false},{"id":462073,"name":"Leila Ghanbari","orcid":"0000-0002-0582-6739","position":8,"is_corresponding":false},{"id":462077,"name":"Suhasa B. Kodandaramaiah","orcid":"0000-0002-7767-2644","position":9,"is_corresponding":false},{"id":556333,"name":"Timothy J. Ebner","orcid":"0000-0001-8464-3860","position":10,"is_corresponding":false},{"id":556329,"name":"Sarah L. West","orcid":"0000-0002-4438-0979","position":0,"is_corresponding":true}],"reference_count":101,"raw_metadata":null,"created_at":"2026-07-18T23:14:13.002105Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}