{"doi":"10.1101/2020.07.01.182105","title":"eEF3 promotes late stages of tRNA translocation on the ribosome","abstract":"Summary In addition to the conserved translation elongation factors eEF1A and eEF2, fungi require a third essential elongation factor, eEF3. While eEF3 has been implicated in tRNA binding and release at the A and E sites, its exact mechanism of action is unclear. Here we show that eEF3 acts at the mRNA–tRNA translocation step by promoting the dissociation of the tRNA from the E site, but independent of aminoacyl-tRNA recruitment to the A site. Depletion of eEF3 in vivo leads to a general slow-down in translation elongation due to accumulation of ribosomes with an occupied A site. Cryo-EM analysis of ex vivo eEF3-ribosome complexes shows that eEF3 facilitates late steps of translocation by favoring non-rotated ribosomal states as well as by opening the L1 stalk to release the E-site tRNA. Additionally, our analysis provides structural insights into novel translation elongation states, enabling presentation of a revised yeast translation elongation cycle.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123851,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9503,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":568053,"name":"Agnieszka A. Pochopien","orcid":null,"position":1,"is_corresponding":false},{"id":225951,"name":"Colin Chih‐Chien Wu","orcid":"0000-0002-6013-0224","position":2,"is_corresponding":false},{"id":351886,"name":"Bertrand Beckert","orcid":"0000-0002-9507-3127","position":3,"is_corresponding":false},{"id":567393,"name":"Sandra Blanchet","orcid":"0000-0001-7804-7739","position":4,"is_corresponding":false},{"id":17827,"name":"Rachel Green","orcid":"0000-0001-9337-2003","position":5,"is_corresponding":false},{"id":522399,"name":"Marina V. Rodnina","orcid":"0000-0003-0105-3879","position":6,"is_corresponding":false},{"id":17892,"name":"Daniel N. Wilson","orcid":"0000-0003-3816-3828","position":7,"is_corresponding":false},{"id":567392,"name":"Namit Ranjan","orcid":"0000-0002-6032-0319","position":0,"is_corresponding":true}],"reference_count":91,"raw_metadata":null,"created_at":"2026-07-18T23:15:03.403566Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}